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Variation within the glycoprotein B gene of human cytomegalovirus is due to homologous recombination
Ist Teil von
Journal of general virology, 1999-06, Vol.80 (6), p.1495-1500
Ort / Verlag
England: Soc General Microbiol
Erscheinungsjahr
1999
Quelle
MEDLINE
Beschreibungen/Notizen
M Haber, U Meyer-Konig and FT Hufert
Abteilung Virologie, Institut fur Medizinische Mikrobiologie und Hygiene der Universitat Freiburg, Hermann-Herder-Str. 11, 79104 Freiburg, Germany
Human cytomegalovirus (HCMV) strains can be classified into different
glycoprotein B (gB) genotypes. In a previous study, frequent intragenic
variation of the gB gene was shown. The aim of this study was to analyse
whether gB variation was due to homologous recombination. The gB gene of
DNA extracts derived from the peripheral blood leukocytes of 14
immunosuppressed patients was amplified by PCR and cloned. Three variable
sites of gB were analysed by restriction fragment analysis and DNA
sequencing and compared with published prototypic strains. In three
patients doubly infected with two distinct HCMV gB strains, prototypic
(60--85%) and non-prototypic recombinant strains (5--40%) were detected. To
demonstrate that homologous recombination is driving HCMV gB variability,
cells were coinfected with plaque-purified prototypic gB strains and
recombinant gB genes were selectively amplified by PCR. gB recombinants
were detected after 15 days of coculture and cross-over sites were
determined by sequencing. These data indicate that homologous recombination
contributes to the variability of the gB gene in vitro and in vivo.