Details

Autor(en) / Beteiligte

• Banciu, Horia L.
• Sorokin, Dimitry Y.
• Tourova, Tatyana P.
• Galinski, Erwin A.
• Muntyan, Maria S.
• Kuenen, J. Gijs
• Muyzer, Gerard

Titel

Influence of salts and pH on growth and activity of a novel facultatively alkaliphilic, extremely salt-tolerant, obligately chemolithoautotrophic sufur-oxidizing Gammaproteobacterium Thioalkalibacter halophilus gen. nov., sp. nov. from South-Western Siberian soda lakes

Ist Teil von

• Extremophiles : life under extreme conditions, 2008-05, Vol.12 (3), p.391-404

Ort / Verlag

Japan: Springer Japan

Erscheinungsjahr

2008

Link zum Volltext

Quelle

SpringerLink

Beschreibungen/Notizen

• A chemolithoautotrophic sulfur-oxidizing bacterium (SOB) strain ALCO 1 capable of growing at both near-neutral and extremely alkaline pH was isolated from hypersaline soda lakes in S-W Siberia (Altai, Russia). Strain ALCO 1 represents a novel separate branch within the halothiobacilli in the Gammaproteobacteria , which, so far, contained only neutro-halophilic SOB. On the basis of its unique phenotypic properties and distant phylogeny, strain ALCO 1 is proposed as a new genus and species Thioalkalibacter halophilus gen. nov. sp. nov. ALCO 1 was able to grow within a broad range of salinity (0.5–3.5 M of total sodium) with an optimum at around 1 M Na + , and pH (7.2–10.2, pH opt at around 8.5). Na + was required for sulfur-dependent respiration in ALCO 1. The neutral (NaCl)-grown chemostat culture had a much lower maximum growth rate (μ max ), respiratory activity and total cytochrome c content than its alkaline-grown counterpart. The specific concentration of osmolytes (ectoine and glycine-betaine) produced at neutral pH and 3 M NaCl was roughly two times higher than at pH 10 in soda. Altogether, strain ALCO 1 represents an interesting chemolithoautotrophic model organism for comparative investigations of bacterial adaptations to high salinity and pH.