Details

Autor(en) / Beteiligte

• Xing, Hui-Chun
• Li, Lan-Juan
• Xu, Kai-Jin
• Shen, Tian
• Chen, Yun-Bo
• Sheng, Ji-Fang
• Chen, Yu
• Fu, Su-Zhen
• Chen, Chun-Lei
• Wang, Jian-Guo
• Yan, Dong
• Dai, Fang-Wei
• Zheng, Shu-Sen

Titel

Protective role of supplement with foreign Bifidobacterium and Lactobacillus in experimental hepatic ischemia-reperfusion injury

Ist Teil von

• Journal of gastroenterology and hepatology, 2006-04, Vol.21 (4), p.647-656

Ort / Verlag

Melbourne, Australia: Blackwell Publishing Asia

Erscheinungsjahr

2006

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Background and Aim:  Intestinal microflora play a crucial role in some severe liver diseases. The purpose of this study was to evaluate the effects of a Lactobacillus strain and a Bifidobacterium strain on ischemia‐reperfusion (I/R) liver injury. Methods:  Rats were divided into six groups. Each group received either Bifidobacterium Catenulatum ZYB0401; Lactobacillus Fermentum ZYL0401; a mixture of these two bacterial strains; gentamicin; or saline by daily gavage for 7 days. On the sixth day, all rats, except those in the control group, were subjected to 20 min of liver ischemia. After 22 h of hepatic reperfusion, liver enzymes and histology, malondialdehyde (MDA), superoxide dismutase (SOD), endotoxemia, serum tumor necrosis factor‐α (TNF‐α), intestinal bacteria, intestinal mucosal ultrastructure, and bacterial translocation were studied. Results:  All administered bacteria increased intestinal Bifidobacterium and Lactobacillus, decreased endotoxemia (P < 0.01), alanine aminotransferase (ALT) (P < 0.01), and markedly ameliorated liver histology and intestinal mucosal ultrastructure. Only rats treated with Bifidobacterium Catenulatum ZYB0401 and Lactobacillus Fermentum ZYL0401 showed reduced incidence of bacterial translocation to the kidney (P < 0.05), associated with decreased serum TNF‐α and liver MDA (P < 0.05) and increased liver SOD (P < 0.05) compared to the I/R group. Gentamicin decreased almost all kinds of intestinal bacteria (P < 0.01) and decreased ALT (P < 0.01) and serum TNF‐α, but failed to reduce both endotoxemia and the incidence of bacterial translocation and had no effects on liver MDA and SOD. Conclusion:  Bifidobacterium Catenulatum ZYB0401 in combination with Lactobacillus Fermentum ZYL0401 could be useful in restoring intestinal microflora and in preventing liver injury in hepatic I/R of rats.