Nature immunology, 2009-10, Vol.10 (10), p.1065-1072
2009
Details
- Autor(en) / Beteiligte
- Titel
- RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate
- Ist Teil von
- Nature immunology, 2009-10, Vol.10 (10), p.1065-1072
- Ort / Verlag
- United States: Nature Publishing Group
- Erscheinungsjahr
- 2009
- Link zum Volltext
- Quelle
- Alma/SFX Local Collection
- Beschreibungen/Notizen
- RNA is sensed by Toll-like receptor 7 (TLR7) and TLR8 or by the RNA helicases LGP2, Mda5 and RIG-I to trigger antiviral responses. Much less is known about sensors for DNA. Here we identify a novel DNA-sensing pathway involving RNA polymerase III and RIG-I. In this pathway, AT-rich double-stranded DNA (dsDNA) served as a template for RNA polymerase III and was transcribed into double-stranded RNA (dsRNA) containing a 5'-triphosphate moiety. Activation of RIG-I by this dsRNA induced production of type I interferon and activation of the transcription factor NF-kappaB. This pathway was important in the sensing of Epstein-Barr virus-encoded small RNAs, which were transcribed by RNA polymerase III and then triggered RIG-I activation. Thus, RNA polymerase III and RIG-I are pivotal in sensing viral DNA.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1529-2908eISSN: 1529-2916DOI: 10.1038/ni.1779Titel-ID: cdi_proquest_miscellaneous_67660901
- Format
- –
- Schlagworte
- Animals, Biological response modifiers, DEAD Box Protein 58, DEAD-box RNA Helicases - immunology, DEAD-box RNA Helicases - metabolism, Deoxyribonucleic acid, DNA, DNA, Viral - immunology, DNA, Viral - metabolism, DNA-directed RNA polymerase, Double-stranded RNA, Enzyme-Linked Immunosorbent Assay, Epstein-Barr virus, Flow Cytometry, Health aspects, Helicases, Humans, Immune response, Immune system, Interferon, Interferon Type I - biosynthesis, Intermediates, Leukocytes, Mononuclear - immunology, Leukocytes, Mononuclear - metabolism, Mice, NF-kappa B - immunology, NF-kappa B - metabolism, NF-κB protein, Poly dA-dT - immunology, Poly dA-dT - metabolism, Properties, Receptors, Immunologic, Regulation, Reverse Transcriptase Polymerase Chain Reaction, RNA polymerase, RNA Polymerase III - immunology, RNA Polymerase III - metabolism, RNA polymerases, RNA, Double-Stranded - immunology, RNA, Viral - immunology, RNA, Viral - metabolism, TLR7 protein, Toll-like receptors, Transcription activation, Transcription, Genetic