Details

Autor(en) / Beteiligte

• Tam, Jerry C.H
• Grant, Nicola L., MSc
• Freire-Moran, Laura, MSc
• Tolmie, Helen, PhD
• French, Neil, FRCP, PhD
• Gordon, Stephen B., FRCP, MD

Titel

Opsonic function of bronchoalveolar lavage IgG after pneumococcal vaccination in HIV-infected and uninfected adults

Ist Teil von

• Journal of allergy and clinical immunology, 2009-06, Vol.123 (6), p.1420-1421

Ort / Verlag

United States: Mosby, Inc

Erscheinungsjahr

2009

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• To the Editor: Pneumococcal conjugate vaccination is immunogenic both systemically and at the mucosal surface,1 reducing carriage and disease, thus resulting in herd immunity.2 Pneumococcal conjugate vaccination is effective at preventing disease in HIV-infected adults,3 despite impairments in antipneumococcal opsonic function in both serum4 and bronchoalveolar lavage (BAL).5 We wished to determine whether improvement in the opsonic function of IgG in BAL could be detected after vaccination. BAL was centrifuged, the supernatant was stored at -80°C, and the concentrations of IgG against each of the pneumococcal serotypes were confirmed by ELISA.1 The opsonic function of BAL IgG was assessed as previously described.5 Briefly, BAL IgG was concentrated 10-fold by using IgG affinity chromatography columns (Pierce Biotechnology, Rockford, Ill).