FEMS immunology and medical microbiology, 2009-04, Vol.55 (3), p.404-413
2009
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- Autor(en) / Beteiligte
- Titel
- Evaluation of colostrum-derived human mammary-associated serum amyloid A3 (M-SAA3) protein and peptide derivatives for the prevention of enteric infection: in vitro and in murine models of intestinal disease
- Ist Teil von
- FEMS immunology and medical microbiology, 2009-04, Vol.55 (3), p.404-413
- Ort / Verlag
- Oxford, UK: Oxford, UK : Blackwell Publishing Ltd
- Erscheinungsjahr
- 2009
- Quelle
- Wiley-Blackwell Journals
- Beschreibungen/Notizen
- In vitro experiments confirmed that a 10-mer peptide derived from human mammary-associated serum amyloid A3 (M-SAA3) protected intestinal epithelial cells from enteropathogenic Escherichia coli (EPEC) adherence. The entire 42-mer human M-SAA3 protein was even more effective, reducing EPEC binding by 72% relative to untreated cells (P<0.05), compared with 25% and 57% reductions for the human 10-mer and Lactobacillus GG, respectively. However, none of the M-SAA3 peptides reduced Salmonella invasion in vitro (P>0.05). Each of the M-SAA3 10-mer peptides and the 42-mer was then administered orally to mice at 500 μg day⁻¹ for 4 days before deliberate infection with either Citrobacter rodentium (mouse model of EPEC) or Salmonella Typhimurium. None of the peptides protected against Salmonella infection and the 42-mer may even increase infection, as there was a trend towards increased Salmonella counts in the liver and small intestine in 42-mer-treated mice compared with those in sodium acetate-treated control mice. Citrobacter counts were reduced in the caecum of mice administered the 42-mer relative to a scrambled 10-mer (P<0.05), but not compared with the sodium acetate control and no reductions were observed in the faeces or colon. Overall, although promising anti-infective activity was demonstrated in vitro, neither the 42-mer M-SAA3 protein nor a 10-mer peptide derivative prevented enteric infection in the animal models tested.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0928-8244eISSN: 1574-695X, 2049-632XDOI: 10.1111/j.1574-695X.2009.00539.xTitel-ID: cdi_proquest_miscellaneous_67039089
- Format
- –
- Schlagworte
- Acetic acid, Amyloid, Animal models, Animals, Antiinfectives and antibacterials, Bacterial Adhesion - drug effects, Bacteriology, Biological and medical sciences, Cecum - microbiology, Cell Line, Citrobacter, Citrobacter rodentium, Citrobacter rodentium - drug effects, Colon, Colon - microbiology, Colostrum, E coli, Enteritis - prevention & control, Enterobacteriaceae Infections - prevention & control, enteropathogenic Escherichia coli, Epithelial cells, Escherichia coli, Escherichia coli - drug effects, Feces - microbiology, Fundamental and applied biological sciences. Psychology, Humans, infection, Infections, Intestine, Intestine, Small - microbiology, Lactobacilli, Lactobacillus, Lactobacillus rhamnosus, Liver - microbiology, mammary associated serum amyloid A3, Mice, Microbiology, Miscellaneous, mouse, Oral administration, Peptides, Peptides - chemical synthesis, Peptides - immunology, Peptides - isolation & purification, Proteins, Salmonella, Salmonella typhimurium, Salmonella typhimurium - drug effects, Serum Amyloid A Protein - chemical synthesis, Serum Amyloid A Protein - immunology, Serum Amyloid A Protein - isolation & purification, Small intestine, Sodium, Sodium acetate