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Main conclusion
Overexpression of
JcSEP3
causes defective stamen development in
Jatropha curcas,
in which brassinosteroid and gibberellin signaling pathways may be involved
.
SEPALLATAs
(
SEPs
), the class E genes of the ABCE model, are required for floral organ determination. In this study, we investigated the role of the
JcSEP3
gene in floral organ development in the woody plant
Jatropha curcas
. Transgenic
Jatropha
plants overexpressing
JcSEP3
displayed abnormal phenotypes such as deficient anthers and pollen, as well as free stamen filaments, whereas
JcSEP3
-RNA interference (RNAi) transgenic plants had no obvious phenotypic changes, suggesting that
JcSEP3
is redundant with other
JcSEP
genes in
Jatropha
. Moreover, we compared the transcriptomes of wild-type plants,
JcSEP3
-overexpressing, and
JcSEP3-
RNAi transgenic plants. In the
JcSEP3
-overexpressing transgenic plants, we discovered 25 upregulated genes involved in anther and pollen development, as well as 12 induced genes in brassinosteroid (BR) and gibberellin (GA) signaling pathways. These results suggest that
JcSEP3
directly or indirectly regulates stamen development, concomitant with the regulation of BR and GA signaling pathways. Our findings help to understand the roles of
SEP
genes in stamen development in perennial woody plants.