Details

Autor(en) / Beteiligte

• Zhang, Xiao‐Jian
• Qi, Feng‐Yu
• Qi, Jia‐Mei
• Yang, Fei
• Shen, Jiang‐Wei
• Cai, Xue
• Liu, Zhi‐Qiang
• Zheng, Yu‐Guo

Titel

Efficient enzymatic synthesis of L‐ascorbyl palmitate using Candida antarctica lipase B‐embedded metal‐organic framework

Ist Teil von

• Biotechnology progress, 2022-01, Vol.38 (1), p.e3218-n/a

Ort / Verlag

Hoboken, USA: John Wiley & Sons, Inc

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Wiley Online Library (Online service)

Beschreibungen/Notizen

• The Candida antarctica lipase B (CALB) was embedded in the metal‐organic framework, zeolitic imidazolate framework‐8 (ZIF‐8), and applied in the enzymatic synthesis of L‐ascorbic acid palmitate (ASP) for the first time. The obtained CALB@ZIF‐8 achieved the enzyme loading of 80 mg g−1 with 11.3 U g−1 (dry weight) unit activity, 59.8% activity recovery, and 92.7% immobilization yield. Under the optimal condition, ASP was synthesized with over 75.9% conversion of L‐ascorbic acid in a 10‐batch reaction. Continuous synthesis of ASP was subsequently performed in a packed bed bioreactor with an outstanding average space–time yield of 58.1 g L−1 h−1, which was higher than ever reported continuous ASP biosynthesis reactions.