Details

Autor(en) / Beteiligte

• Ahmed Zaki, Mohamed Samir
• Haidara, Mohamed A.
• Abdallaa, Asim M.
• Mohammed, Heitham
• Sideeg, Abulqasim M.
• Eid, Refaat A.

Titel

Role of dietary selenium in alleviating bisphenol A toxicity of liver albino rats: Histological, ultrastructural, and biomarker assessments

Ist Teil von

• Journal of food biochemistry, 2021-05, Vol.45 (5), p.e13725-n/a

Ort / Verlag

United States

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• Bisphenol A (BPA) is used as a plasticizer in polycarbonate plastics. It has been used in consumer products and epoxy resins for decades as protective coatings and linings for food and beverage bottles. This can trigger human reactions to BPA which interferes with estrogen receptors. Our study explored the ameliorative effects of selenium (Se) in male rats on liver damage caused by BPA. Rats were divided into four groups at random: The first one obtained olive oil and acted as a control. Se (0.5 mg/kg diet) was given for the second group. The third one was treated with BPA (10 mg/kg body weight/day) orally. Concomitantly Se (0.5 mg/kg diet) and BPA (10 mg/kg body weight/day) were given orally in the fourth one. Liver specimens were prepared for light, electron microscopes and the serum samples were screened for biochemical markers. In the BPA received group, histological findings indicated apoptotic hepatic histological changes such as sinusoidal congestion, cytoplasmic vacuolation and leukocyte infiltration. Ultrastructurally, the same group had mitochondrial degeneration, rough endoplasmic reticulum swelling, and nuclear pyknosis, as well as fat droplet deposition and lysosome enhancement. Liver enzymes: In the BPA group, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) have been substantially increased. Moreover, histological and ultrastructural improvements were seen in the rat population treated with BPA and Se, whereas ALT and AST levels were lowered and malondialdehyde (MDA), glutathione peroxidase (GPx), human C reactive protein (hCRP), and the serum levels of interleukin‐6 (IL‐6) were significantly modulated. Practical applications Bisphenol A (BPA) is used in the manufacturing of polycarbonate plastic (e.g., water bottles, baby bottles) and epoxy resins (e.g., inner coating in metallic food cans). It is a non‐polymer preservative for other plastics, one of the contaminants of the atmosphere and a common endocrine estrogenic disruptor. Our study explored the ameliorative effects of selenium (Se) in male rats on liver damage caused by BPA. Rats were divided into four groups at random: The first one obtained olive oil and acted as a control. Se (0.5 mg/kg diet) was given for the second group. The third one was treated with BPA (10 mg/kg body weight/day) orally. Concomitant Se (0.5 mg/kg diet) and BPA (10 mg/kg body weight/day) were given in the fourth one. Liver specimens were prepared for light, electron microscopes and the serum samples were screened for biochemical markers. Apoptotic hepatic histological changes were observed in the Bisphenol‐A (BPA) treated population, along with ultrastructural changes in most cytoplasmic organelles and nuclear pyknosis. The levels of the liver enzymes ALT and AST have greatly increased.Furthermore, the rat population treated with BPA and selenium (Se) showed histological and ultrastructural improvements in liver sections, while ALT and AST levels were reduced and MDA, GPx, hCRP, and IL‐6 levels were significantly modulated. So, Se's hepatoprotective role against BPA exposure has been confirmed.