Details

Autor(en) / Beteiligte

• Hipp, Katharina
• Zikeli, Kerstin
• Kepp, Gabi
• Schmid, Lena
• Shoeman, Robert L.
• Jurkowski, Tomasz P.
• Kleinow, Tatjana
• Jeske, Holger

Titel

Different forms of African cassava mosaic virus capsid protein within plants and virions

Ist Teil von

• Virology (New York, N.Y.), 2019-03, Vol.529, p.81-90

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2019

Link zum Volltext

Quelle

ScienceDirect Journals (5 years ago - present)

Beschreibungen/Notizen

• One geminiviral gene encodes the capsid protein (CP), which can appear as several bands after electrophoresis depending on virus and plant. African cassava mosaic virus-Nigeria CP in Nicotiana benthamiana, however, yielded one band (~ 30 kDa) in total protein extracts and purified virions, although its expression in yeast yielded two bands (~ 30, 32 kDa). Mass spectrometry of the complete protein and its tryptic fragments from virions is consistent with a cleaved start M1, acetylated S2, and partial phosphorylation at T12, S25 and S62. Mutants for additional potentially modified sites (N223A; C235A) were fully infectious and formed geminiparticles. Separation in triton acetic acid urea gels confirmed charge changes of the CP between plants and yeast indicating differential phosphorylation. If the CP gene alone was expressed in plants, multiple bands were observed like in yeast. A high turnover rate indicates that post-translational modifications promote CP decay probably via the ubiquitin-triggered proteasomal pathway. •ACMV CP yields a single band in SDS-PAGE throughout a viral infection in plants.•Without a viral infection, the CP expressed in plants yields multiple bands.•Forms of the CP with slower migration react with an anti-ubiquitin antibody.•Plant CP is phosphorylated partially at three positions within the N-terminus.•C-terminal mutants are fully infectious and form geminate virus particles.