Details

Autor(en) / Beteiligte

• Kumthip, Kattareeya
• Khamrin, Pattara
• Ushijima, Hiroshi
• Maneekarn, Niwat

Titel

Molecular epidemiology of classic, MLB and VA astroviruses isolated from <5 year-old children with gastroenteritis in Thailand, 2011–2016

Ist Teil von

• Infection, genetics and evolution, 2018-11, Vol.65, p.373-379

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2018

Link zum Volltext

Quelle

ScienceDirect Journals (5 years ago - present)

Beschreibungen/Notizen

• This study aimed to investigate the molecular epidemiology and genetic diversity of astrovirus circulating in pediatric patients with acute gastroenteritis in Thailand. A total of 2034 fecal specimens collected between 2011 and 2016 from children hospitalized with acute gastroenteritis were analyzed for astrovirus infection using RT-PCR and nucleotide sequencing techniques. Overall, 54 samples were positive for astrovirus (2.6%). About half of the virus-positive samples (57.4%) were co-infection cases, mainly with rotavirus or norovirus. Genotyping revealed that three major clades, classic human astrovirus (HAstV) and newly emerging MLB and VA astroviruses co-circulated in the study population. At least seven different genotypes were identified and HAstV1 was the most predominant genotype detected (50%), followed by MLB1 (16.7%), HAstV4 (11.1%), HAstV5 and HAstV8 (7.4% each), MLB2 and VA2 (3.7% each). This study provided a useful information and contributed to molecular epidemiology of astroviruses including the novel MLB and VA astoviruses have been characterized and reported for the first time in Thailand. •2034 fecal specimens from children with diarrhea were tested for astrovirus infection by RT-PCR in Thailand, 2011-2016.•The overall prevalence of astroviruses during the observation period was 2.6%.•Astrovirus detection comprised HAstVs, MLB and VA astroviruses.•HAstV1 was the most predominant genotype; seven different AstV genotypes were identified.