Details

Autor(en) / Beteiligte

• Klotz, Luisa
• Dani, Indra
• Edenhofer, Frank
• Nolden, Lars
• Evert, Bernd
• Paul, Bianca
• Kolanus, Waldemar
• Klockgether, Thomas
• Knolle, Percy
• Diehl, Linda

Titel

Peroxisome Proliferator-Activated Receptor gamma Control of Dendritic Cell Function Contributes to Development of CD4 super(+) T Cell Anergy

Ist Teil von

• Journal of Immunology, 2007-02, Vol.178 (4), p.2122-2131

Erscheinungsjahr

2007

Link zum Volltext

Quelle

Wiley Online Library Journals Frontfile Complete

Beschreibungen/Notizen

• There is increasing evidence that dendritic cell (DC) immunogenicity is not only positively regulated by ligands of pattern recognition receptors, but also negatively by signals that prevent DC activation and full functional maturation. Depending on their activation status, DCs can induce either immunity or tolerance. In this study, we provide molecular evidence that the transcription factor peroxisome proliferator-activated receptor gamma (PPAR gamma ) is a negative regulator of DC maturation and function. Sustained PPAR gamma activation in murine DCs reduced maturation-induced expression of costimulatory molecules and IL-12, and profoundly inhibited their capacity to prime naive CD4 super(+) T cells in vitro. Using PPAR gamma -deficient DCs, generated by Cre-mediated ablation of the PPAR gamma gene, agonist-mediated suppression of maturation-induced functional changes were abrogated. Moreover, absence of PPAR gamma increased DC immunogenicity, suggesting a constitutive regulatory function of PPAR gamma in DCs. Adoptive transfer of PPAR gamma -activated Ag-presenting DCs induced CD4 super(+) T cell anergy, characterized by impaired differentiation resulting in absent Th1 and Th2 cytokine production and failure of secondary clonal expansion upon restimulation. Collectively, our data support the notion that PPAR gamma is an efficient regulator of DC immunogenicity that may be exploited to deliberately target CD4 super(+) T cell-mediated immune responses.