Details

Autor(en) / Beteiligte

• Asahi-Ozaki, Yasuko
• Itamura, Shigeyuki
• Ichinohe, Takeshi
• Strong, Peter
• Tamura, Shin-ichi
• Takahashi, Hidehiro
• Sawa, Hirofumi
• Moriyama, Masami
• Tashiro, Masato
• Sata, Tetsutaro
• Kurata, Takeshi
• Hasegawa, Hideki

Titel

Intranasal administration of adjuvant-combined recombinant influenza virus HA vaccine protects mice from the lethal H5N1 virus infection

Ist Teil von

• Microbes and infection, 2006-10, Vol.8 (12), p.2706-2714

Ort / Verlag

Lausanne: Elsevier SAS

Erscheinungsjahr

2006

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• Attenuated recombinant H5N1 influenza virus was constructed to develop a safe H5N1 influenza vaccine. The immunogenicity and protective effect of the vaccine prepared from haemagglutinin-modified recombinant H5N1 influenza virus was evaluated in mice intranasally co-administered with cholera toxin B subunit containing a trace amount of holotoxin (CTB*), synthetic double-stranded RNA, poly (I:C) or chitin microparticles (CMP) as adjuvants. Intranasal administration of recombinant H5 HA split vaccine with CTB* or poly(I:C) and/or CMP elicited an immunological response with both anti-H5 HA IgA in the nasal wash and anti-H5 HA IgG antibody in the serum, and showed a protective against lethal H5N1 A/Hong Kong/483/97 (HK483) infection. We also demonstrated that intranasal co-administration of antigen with both poly (I:C) and CMP enhanced the expression of Toll-like receptor (TLR) 3, TLR7 in the spleen. These results indicate that poly (I:C) and CMP are highly effective as mucosal adjuvants for use with the nasal H5N1 vaccine.