Details

Autor(en) / Beteiligte

• Clemmensen, Christoffer
• Jørgensen, Christinna V.
• Smajilovic, Sanela
• Bräuner‐Osborne, Hans

Titel

Robust GLP‐1 secretion by basic L‐amino acids does not require the GPRC6A receptor

Ist Teil von

• Diabetes, obesity & metabolism, 2017-04, Vol.19 (4), p.599-603

Ort / Verlag

Oxford, UK: Blackwell Publishing Ltd

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• The G protein‐coupled receptor GPRC6A (GPCR, Class C, group 6, subtype A) has been proposed to be a sensor for basic L‐amino acids that are hypothesized to translate ingestive behaviour to endocrine information. However, the contribution of the GPRC6A receptor to L‐amino acid‐induced glucagon‐like peptide 1 (GLP‐1) secretion is unclear. Therefore, to discover whether the GPRC6A receptor is indispensible for amino acid‐induced secretion of GLP‐1, we treated, with oral gavage, GPRC6A knock‐out (KO) and wild‐type (WT) littermate mice with GPRC6A ligands (L‐arginine and L‐ornithine) and assessed GLP‐1 levels in circulation. We found that oral administration of both L‐arginine and L‐ornithine significantly increased total plasma GLP‐1 levels to a similar level in GPRC6A KO and WT mice 15 minutes after gavage (both amino acids) and accumulated up to 60 minutes after gavage (L‐arginine). Conversely, GLP‐1 secretion at the 30‐ and 60‐minute time points in the KO mice was attenuated and did not reach statistical significance. In summary, these data confirm that L‐arginine is a potent GLP‐1 secretagogue and show that the main effect occurs independently of GPRC6A. In addition, this is the first study to show that also L‐ornithine powerfully elicits GLP‐1 release in vivo.