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Identification of bacterial pathogens from formalin-fixed paraffin-embedded tissues by using 16S sequencing: Retrospective correlation of results to clinicians' responses
Ist Teil von
Human pathology, 2017-01, Vol.59, p.132-138
Ort / Verlag
United States: Elsevier Inc
Erscheinungsjahr
2017
Link zum Volltext
Quelle
Elsevier ScienceDirect Journals Complete
Beschreibungen/Notizen
Summary 16S sequencing on formalin-fixed paraffin-embedded (FFPE) material has been used to identify bacteria when culture-based phenotyping techniques have not worked. The objective of this study was to determine how frequently 16S sequencing used in FFPE material was helpful to clinicians in the diagnosis and treatment of infectious diseases. Requests for testing occurred upon consultation between an infectious disease pathologist and a surgical pathologist or an infectious disease physician. A selected paraffin block from each case was referred for 16S sequencing. Retrospectively, we correlated clinical history and management decisions on 27 cases that were tested by paneubacterial 16S sequencing. Samples included 24 surgical specimens, 1 autopsy, and 2 cytology blocks. Seventeen of the 27 cases (63%) had a positive 16S sequencing. Acute inflammation was present in ten of these cases and organisms were observed using special stains in three. In 11 of the 17 cases (65%) clinicians considered the organism identified by 16S sequencing to be the cause or possible cause of the infectious process. Organisms included common ( Citrobacter ) and fastidious bacteria ( Haemophilus, Fusobacterium ). In 3 cases clinicians changed antibiotic treatment based on the bacteria identified, while in 8 (including 2 where no organism was found) clinicians continued the antibiotic treatment. The use of 16S sequencing on FFPE identified specific bacteria even when organisms were not observed histopathologically. 16S results had an impact in infectious disease management decisions.