Details

Autor(en) / Beteiligte

• Cuniasse, Philippe
• Tavares, Paulo
• Orlova, Elena V
• Zinn-Justin, Sophie

Titel

Structures of biomolecular complexes by combination of NMR and cryoEM methods

Ist Teil von

• Current opinion in structural biology, 2017-04, Vol.43, p.104-113

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2017

Link zum Volltext

Quelle

ScienceDirect Journals (5 years ago - present)

Beschreibungen/Notizen

• •CryoEM combined with NMR provided atomic models of biocomplexes.•NMR structures together with X-ray structures enabled interpretation of cryoEM maps.•Solid-state NMR and cryoEM resolved structures of helical filaments and fibrils.•These approaches validated and improved models built de novo using cryoEM maps.•NMR structures were compared with cryoEM models to uncover assembly mechanisms. CryoEM is presently providing structures of biocomplexes considered intractable to analysis by other structural techniques. NMR is playing an important role in delivering structural information on dynamics events and conformational heterogeneity. Impressive results were obtained by combining cryoEM and either liquid- or solid-state NMR, revealing the structures of cellular machines, filaments and amyloid fibrils. NMR solution structures of proteins and nucleic acids were fitted, together with crystallographic structures, into cryoEM maps of large complexes, to decipher their assembly mechanisms and describe their functional dynamics. Modelling based on solid-state NMR and cryoEM data provided 3D structure of filaments and fibrils. These NMR approaches validated, but also corrected, atomic models built de novo in cryoEM maps, and provided new structural data on flexible or structurally heterogeneous systems. Combination of cryoEM and NMR became an established hybrid approach in structural biology that significantly contributes to our understanding of functional mechanisms in supramolecular assemblies.