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Expression of the anti-inflammatory suppressor of cytokine signaling 3 (SOCS3) in human clear cell renal cell carcinoma
Ist Teil von
Tumor biology, 2016-07, Vol.37 (7), p.9649-9656
Ort / Verlag
Dordrecht: Springer Netherlands
Erscheinungsjahr
2016
Link zum Volltext
Quelle
Springer Nature - Complete Springer Journals
Beschreibungen/Notizen
The oncogenic transcription factor signal transducer and activator of transcription 3 (STAT3) is a cytokine-activated transcription factor controlling inflammation, cell proliferation, survival, and differentiation in normal tissue as well as in tumor growth. One of its most important negative regulators is the suppressor of cytokine signaling 3 (
SOCS3
). Here, we analyzed
SOCS3
and other tumor-associated local immune regulators in human clear cell renal cell carcinoma (ccRCC). Analyses were performed in tumor and adjacent tumor-free healthy renal tissue from 35 patients with ccRCC. For functional analysis, ccRCC Caki-1 cell lines were stimulated with IL-6 and IFNγ in cell culture assays. We observed significantly lower
SOCS3
messenger RNA (mRNA) levels in tumor tissue compared to healthy tissue.
SOCS3
mRNA strongly correlated within tumor and healthy tissue. Interestingly vice versa,
SOCS3
protein levels were significantly higher in tumor tissue than in healthy tissue.
IL-22
and
IL-22R1
mRNA displayed no differences in tumor and healthy tissue. Stimulation of Caki-1 cells with IFNγ resulted in markedly increased
SOCS3
mRNA levels. We conclude that SOCS3 along with STAT3 participates in regulatory mechanisms in ccRCC, which certainly features only one of multiple factors involved but nevertheless merits further attention.