Details

Autor(en) / Beteiligte

• Chen, Yiqiang
• Chen, Qian
• Han, Miaomiao
• Liu, Jiangyang
• Zhao, Peng
• He, Lidong
• Zhang, Yuan
• Niu, Yiming
• Yang, Wenjun
• Zhang, Liying

Titel

Near-infrared fluorescence-based multiplex lateral flow immunoassay for the simultaneous detection of four antibiotic residue families in milk

Ist Teil von

• Biosensors & bioelectronics, 2016-05, Vol.79, p.430-434

Ort / Verlag

England: Elsevier B.V

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• In this study, we developed a novel near-infrared fluorescence based multiplex lateral flow immunoassay by conjugating a near-infrared label to broad-specificity monoclonal antibody/receptor as detection complexes. Different antigens were dispensed onto separate test zones of nitrocellulose membrane to serve as capture reagents. This assay format allowed the simultaneous detection of four families of antibiotics (β-lactams, tetracyclines, quinolones and sulfonamides) in milk within 20min. Qualitative and quantitative analysis of target antibiotics were realized by imaging the fluorescence intensity of the near-infrared label captured on respective test lines. For qualitative analysis, the cut-off values of β-lactams, tetracyclines, quinolones and sulfonamides were determined to be 8ng/mL, 2ng/mL, 4ng/mL and 8ng/mL respectively, which were much lower than the conventional gold nanoparticle based lateral flow immunoassay. For quantitative analysis, the detection ranges were 0.26–3.56ng/mL for β-lactams, 0.04–0.98ng/mL for tetracyclines, 0.08–2.0ng/mL for quinolones, and 0.1–3.98ng/mL for sulfonamides, with linear correlation coefficients higher than 0.97. The mean spiked recoveries ranged from 93.7% to 108.2% with coefficient of variations less than 16.3%. These results demonstrated that this novel immunoassay is a promising approach for rapidly screening the four families of antibiotic residues in milk. •A near-infrared fluorescence-based immunoassay for small molecules was firstly reported.•This immunoassay can simultaneously detect four families of antibiotic residues in milk.•The assay sensitivity was far below the maximum residue limits set by the European Union.•The whole assay procedure can be completed within 20 min.