Details

Autor(en) / Beteiligte

• Tian, Qiang
• Stepaniants, Serguei B
• Mao, Mao
• Weng, Lee
• Feetham, Megan C
• Doyle, Michelle J
• Yi, Eugene C
• Dai, Hongyue
• Thorsson, Vesteinn
• Eng, Jimmy
• Goodlett, David
• Berger, Joel P
• Gunter, Bert
• Linseley, Peter S
• Stoughton, Roland B
• Aebersold, Ruedi
• Collins, Steven J
• Hanlon, William A
• Hood, Leroy E

Titel

Integrated Genomic and Proteomic Analyses of Gene Expression in Mammalian Cells

Ist Teil von

• Molecular & cellular proteomics, 2004-10, Vol.3 (10), p.960-969

Ort / Verlag

United States: American Society for Biochemistry and Molecular Biology

Erscheinungsjahr

2004

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Using DNA microarrays together with quantitative proteomic techniques (ICAT reagents, two-dimensional DIGE, and MS), we evaluated the correlation of mRNA and protein levels in two hematopoietic cell lines representing distinct stages of myeloid differentiation, as well as in the livers of mice treated for different periods of time with three different peroxisome proliferative activated receptor agonists. We observe that the differential expression of mRNA (up or down) can capture at most 40% of the variation of protein expression. Although the overall pattern of protein expression is similar to that of mRNA expression, the incongruent expression between mRNAs and proteins emphasize the importance of posttranscriptional regulatory mechanisms in cellular development or perturbation that can be unveiled only through integrated analyses of both proteins and mRNAs.