Details

Autor(en) / Beteiligte

• Bigley, Andrew N
• Xiang, Dao Feng
• Ren, Zhongjie
• Xue, Haoran
• Hull, Kenneth G
• Romo, Daniel
• Raushel, Frank M

Titel

Chemical Mechanism of the Phosphotriesterase from Sphingobium sp. Strain TCM1, an Enzyme Capable of Hydrolyzing Organophosphate Flame Retardants

Ist Teil von

• Journal of the American Chemical Society, 2016-03, Vol.138 (9), p.2921-2924

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2016

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The mechanism of action of the manganese-dependent phosphotriesterase from Sphingobium sp. strain TCM1 that is capable of hydrolyzing organophosphate flame retardants was determined. The enzyme was shown to hydrolyze the R P-enantiomer of O-methyl O-cyclohexyl p-nitrophenyl thiophosphate with net inversion of configuration and without the formation of a covalent reaction intermediate. These results demonstrate that the enzyme catalyzes the hydrolysis of substrates by activation of a nucleophilic water molecule for direct attack at the phosphorus center.