Biotechnology advances, 2015-05, Vol.33 (3-4), p.358-369
2015
Details
- Autor(en) / Beteiligte
- Titel
- Recombinant CBM-fusion technology — Applications overview
- Ist Teil von
- Biotechnology advances, 2015-05, Vol.33 (3-4), p.358-369
- Ort / Verlag
- England: Elsevier Inc
- Erscheinungsjahr
- 2015
- Link zum Volltext
- Quelle
- Elsevier ScienceDirect Journals Complete
- Beschreibungen/Notizen
- Carbohydrate-binding modules (CBMs) are small components of several enzymes, which present an independent fold and function, and specific carbohydrate-binding activity. Their major function is to bind the enzyme to the substrate enhancing its catalytic activity, especially in the case of insoluble substrates. The immense diversity of CBMs, together with their unique properties, has long raised their attention for many biotechnological applications. Recombinant DNA technology has been used for cloning and characterizing new CBMs. In addition, it has been employed to improve the purity and availability of many CBMs, but mainly, to construct bi-functional CBM-fused proteins for specific applications. This review presents a comprehensive summary of the uses of CBMs recombinantly produced from heterologous organisms, or by the original host, along with the latest advances. Emphasis is given particularly to the applications of recombinant CBM-fusions in: (a) modification of fibers, (b) production, purification and immobilization of recombinant proteins, (c) functionalization of biomaterials and (d) development of microarrays and probes.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0734-9750eISSN: 1873-1899DOI: 10.1016/j.biotechadv.2015.02.006Titel-ID: cdi_proquest_miscellaneous_1770269198
- Format
- –
- Schlagworte
- Biomaterials, Biomedical materials, Biotechnology, Carbohydrate-binding activity, Carbohydrate-binding modules, Carbohydrates, Carbohydrates - chemistry, CBM applications, Cellulose, Cellulose - genetics, Cellulose - metabolism, Cloning, Enzymes, Heterologous expression systems, Immobilized Proteins - chemistry, Immobilized Proteins - genetics, Proteins, Receptors, Cell Surface - chemistry, Receptors, Cell Surface - genetics, Receptors, Cell Surface - metabolism, Recombinant, Recombinant CBM-fusions, Recombinant Proteins - chemistry, Recombinant Proteins - genetics, Recombinant Proteins - isolation & purification, Substrate Specificity, Surgical implants