Details

Autor(en) / Beteiligte

• Hardy, Melinda Y
• Girardin, Adam
• Pizzey, Catherine
• Cameron, Donald J
• Watson, Katherine A
• Picascia, Stefania
• Auricchio, Renata
• Greco, Luigi
• Gianfrani, Carmen
• La Gruta, Nicole L
• Anderson, Robert P
• Tye-Din, Jason A

Titel

Consistency in Polyclonal T-cell Responses to Gluten Between Children and Adults With Celiac Disease

Ist Teil von

• Gastroenterology (New York, N.Y. 1943), 2015-11, Vol.149 (6), p.1541-1552.e2

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• Background & Aims Developing antigen-specific approaches for diagnosis and treatment of celiac disease requires a detailed understanding of the specificity of T cells for gluten. The existing paradigm is that T-cell lines and clones from children differ from those of adults in the hierarchy and diversity of peptide recognition. We aimed to characterize the T-cell response to gluten in children vs adults with celiac disease. Methods Forty-one children with biopsy-proven celiac disease (median age, 9 years old; 17 male), who had been on strict gluten-free diets for at least 3 months, were given a 3-day challenge with wheat; blood samples were collected and gluten-specific T cells were measured. We analyzed responses of T cells from these children and from 4 adults with celiac disease to a peptide library and measured T-cell receptor bias. We isolated T-cell clones that recognized dominant peptides and assessed whether gluten peptide recognition was similar between T-cell clones from children and adults. Results We detected gluten-specific responses by T cells from 30 of the children with celiac disease (73%). T cells from the children recognized the same peptides that were immunogenic to adults with celiac disease; deamidation of peptides increased these responses. Age and time since diagnosis did not affect the magnitude of T-cell responses to dominant peptides. T-cell clones specific for dominant α- or ω-gliadin peptides from children with celiac disease had comparable levels of reactivity to wheat, rye, and barley peptides as T-cell clones from adults with celiac disease. The α-gliadin–specific T cells from children had biases in T-cell receptor usage similar to those in adults. Conclusions T cells from children with celiac disease recognize similar gluten peptides as T cells from adults with celiac disease. The findings indicate that peptide-based diagnostics and therapeutics for adults may also be used for children.