Details

Autor(en) / Beteiligte

• Cordova, D.
• Benner, E.A.
• Sacher, M.D.
• Rauh, J.J.
• Sopa, J.S.
• Lahm, G.P.
• Selby, T.P.
• Stevenson, T.M.
• Flexner, L.
• Gutteridge, S.
• Rhoades, D.F.
• Wu, L.
• Smith, R.M.
• Tao, Y.

Titel

Anthranilic diamides: A new class of insecticides with a novel mode of action, ryanodine receptor activation

Ist Teil von

• Pesticide biochemistry and physiology, 2006-03, Vol.84 (3), p.196-214

Ort / Verlag

San Diego, CA: Elsevier Inc

Erscheinungsjahr

2006

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals Complete

Beschreibungen/Notizen

• Development of insecticides with unique modes of action is necessary to combat widespread insecticide resistance. A new class of insecticides has been discovered, the anthranilic diamides, that provides exceptional control through action on a novel target, the ryanodine receptor. Anthranilic diamides potently activate this receptor, releasing stored calcium from the sarcoendoplasmic reticulum causing impaired regulation of muscle contraction. Expression of a recombinant Drosophila ryanodine receptor in a lepidopteran cell line confers sensitivity to anthranilic diamides similar to that observed with native receptors. Ligand-binding studies with radiolabeled ryanodine and radiolabeled anthranilic diamide in Periplaneta americana reveal a single, saturable binding site for this chemistry distinct from that of ryanodine. Further, calcium mobilization studies using mammalian cell lines indicate anthranilic diamides exhibit >500-fold differential selectivity toward insect, over mammalian, receptors. Consequently, anthranilic diamides offer a novel pharmacological tool for calcium signaling research in addition to a unique alternative to existing pest-management strategies.