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Tyrosine Photophysics During the Early Stages of β‑Amyloid Aggregation Leading to Alzheimer’s
Ist Teil von
The journal of physical chemistry letters, 2015-08, Vol.6 (15), p.3116-3120
Ort / Verlag
United States: American Chemical Society
Erscheinungsjahr
2015
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
We have monitored the formation of toxic β-amyloid oligomers leading to Alzheimer’s disease by detecting changes in the fluorescence decay of intrinsic tyrosine. A new approach based on the non-Debye model of fluorescence kinetics resolves the complexity of the underlying photophysics. The gradual disappearance of nonmonotonic fluorescence decay rates, at the early stages of aggregation as larger, tighter-packed oligomers are formed, is interpreted in terms of tyrosine–peptide dielectric relaxation influencing the decay. The results demonstrate the potential for a new type of fluorescence lifetime sensing based on dual excited-state/dielectric relaxation, with application across a broad range of biological molecules. The results also reconcile previously conflicting models of protein intrinsic fluorescence decay based on rotamers or dielectric relaxation by illustrating conditions under which both are manifest.