Journal of proteome research, 2014-05, Vol.13 (5), p.2339-2351
2014
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Details
- Autor(en) / Beteiligte
- Titel
- High-Fat Diet Induced Isoform Changes of the Parkinson’s Disease Protein DJ‑1
- Ist Teil von
- Journal of proteome research, 2014-05, Vol.13 (5), p.2339-2351
- Ort / Verlag
- United States: American Chemical Society
- Erscheinungsjahr
- 2014
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Genetic and environmental factors mediate via different physiological and molecular processes a shifted energy balance leading to overweight and obesity. To get insights into the underlying processes involved in energy intake and weight gain, we compared hypothalamic tissue of mice kept on a high-fat or control diet for 10 days by a proteomic approach. Using two-dimensional difference gel electrophoresis in combination with LC–MS/MS, we observed significant abundance changes in 15 protein spots. One isoform of the protein DJ-1 was elevated in the high-fat diet group in three different mouse strains SWR/J, C57BL/6N, and AKR/J analyzed. Large-scale validation of DJ-1 isoforms in individual samples and tissues confirmed a shift in the pattern of DJ-1 isoforms toward more acidic isoforms in several brain and peripheral tissues after feeding a high-fat diet for 10 days. The identification of oxidation of cysteine 106 as well as 2-succinyl modification of the same residue by mass spectrometry not only explains the isoelectric shift of DJ-1 but also links our results to similar shifts of DJ-1 observed in neurodegenerative disease states under oxidative stress. We hypothesize that DJ-1 is a common physiological sensor involved in both nutrition-induced effects and neurodegenerative disease states.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1535-3893eISSN: 1535-3907DOI: 10.1021/pr401157kTitel-ID: cdi_proquest_miscellaneous_1553708827
- Format
- –
- Schlagworte
- Animals, Blotting, Western, Chromatography, Liquid, Diet, High-Fat - adverse effects, Diet, High-Fat - methods, Electrophoresis, Gel, Two-Dimensional, Hypothalamus - metabolism, Isoelectric Point, Male, Mass Spectrometry - methods, Mice, Inbred AKR, Mice, Inbred C57BL, Mice, Inbred Strains, Obesity - etiology, Obesity - metabolism, Oncogene Proteins - chemistry, Oncogene Proteins - genetics, Oncogene Proteins - metabolism, Overweight - etiology, Overweight - metabolism, Parkinson Disease - metabolism, Peroxiredoxins - chemistry, Peroxiredoxins - genetics, Peroxiredoxins - metabolism, Protein Deglycase DJ-1, Protein Isoforms - chemistry, Protein Isoforms - genetics, Protein Isoforms - metabolism, Proteomics - methods, Reverse Transcriptase Polymerase Chain Reaction, Species Specificity