Details

Autor(en) / Beteiligte

• Nabavi, Seyed Fazel
• Habtemariam, Solomon
• Sureda, Antoni
• Hajizadeh Moghaddam, Akbar
• Daglia, Maria
• Naba, Seyed Mohammad

Titel

In Vivo Protective Effects of Gallic Acid Isolated from Peltiphyllum Peltatum Against Sodium Fluoride-Induced Oxidative Stress in Rat Erythrocytes

Ist Teil von

• Arhiv za higijenu rada i toksikologiju, 2013-12, Vol.64 (4), p.553-559

Ort / Verlag

Croatia: Versita

Erscheinungsjahr

2013

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Gallic acid has been identified as an antioxidant component of the edible and medicinal plant Peltiphyllum peltatum. The present study examined its potential protective role against sodium fluoride (NaF)-induced oxidative stress in rat erythrocytes. Oxidative stress was induced by NaF administration through drinking water (1030.675 mg m-3 for one week). Gallic acid at 10 mg kg and 20 mg kg and vitamin C for positive controls (10 mg kg ) were administered daily intraperitoneally for one week prior to NaF administration. Thiobarbituric acid reactive substances, antioxidant enzyme activities (superoxide dismutase and catalase), and the level of reduced glutathione were evaluated in rat erythrocytes. Lipid peroxidation in NaF-exposed rats significantly increased (by 88.8 %) when compared to the control group (p<0.05). Pre-treatment with gallic acid suppressed lipid peroxidation in erythrocytes in a dose-dependent manner. Catalase and superoxide dismutase enzyme activities and glutathione levels were reduced by NaF intoxication by 54.4 %, 63.69 %, and 42 % (p<0.001; vs. untreated control group), respectively. Pre-treatment with gallic acid or vitamin C significantly attenuated the deleterious effects. Gallic acid isolated from Peltiphyllum peltatum and vitamin C mitigated the NaF-induced oxidative stress in rat erythrocytes. Galna kiselina u ranijim je istraživanjima potvrđena kao važna antioksidativna sastavnica jestive i ljekovite biljke Peltiphyllum peltatum. U ovome istraživanju ispitan je njen potencijalni zaštitni učinak protiv oksidativnog stresa izazvanog natrijevim fluoridom (NaF) u eritrocitima štakora. Oksidativni stres izazvan je davanjem NaF eksperimentalnim životinjama putem vode za piće (1030,675 mg m ) tijekom jednog tjedna. Galna kiselina životinjama je davana intraperitonealno u dozama 10 mg kg i 20 mg kg na dan. Životinje iz pozitivno kontrolne skupine svaki su dan, osim NaF, intraperitonealno primale vitamin C u dozi 10 mg kg . U eritrocitima štakora primjenom TBARS-testa izmjerene su razine lipidne peroksidacije, aktivnosti antioksidativnih enzima (superoksid dismutaze i katalaze) te razina reduciranoga glutationa. Lipidna peroksidacija u štakora izloženih NaF osjetno se povećala (88,8 %) u usporedbi s kontrolnom skupinom (p<0,05). Prethodni tretman galnom kiselinom smanjio je razinu lipidne peroksidacije u eritrocitima, ovisno o dozi. Toksičnost NaF smanjila je aktivnost enzima katalaze i superoksid dismutaze i razine glutationa za 54,4 %, 63,69 %, odnosno 42 % (p<0,001; u usporedbi s kontrolnom skupinom). Dobiveni rezultati upućuju na pozitivne učinke predobrade štakora galnom kiselinom izoliranom iz Peltiphyllum peltatum i vitaminom C na razinu oksidativnog stresa izazvanog natrijevim fl uoridom u eritrocitima štakora.