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Understanding the structural complexity of dissolved organic matter: isomeric diversity
Ist Teil von
Faraday discussions, 2019-08, Vol.218, p.431-44
Ort / Verlag
England: Royal Society of Chemistry
Erscheinungsjahr
2019
Link zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
In the present work, the advantages of ESI-TIMS-FT-ICR MS to address the isomeric content of dissolved organic matter are studied. While the MS spectra allowed the observation of a high number of peaks (
e.g.
, PAN-L: 5004 and PAN-S: 4660), over 4× features were observed in the IMS-MS domain (
e.g.
, PAN-L: 22 015 and PAN-S: 20 954). Assuming a total general formula of C
x
H
y
N
0-3
O
0-19
S
0-1
, 3066 and 2830 chemical assignments were made in a single infusion experiment for PAN-L and PAN-S, respectively. Most of the identified chemical compounds (∼80%) corresponded to highly conjugated oxygen compounds (O
1
-O
20
). ESI-TIMS-FT-ICR MS provided a lower estimate of the number of structural and conformational isomers (
e.g.
, an average of 6-10 isomers per chemical formula were observed). Moreover, ESI-q-FT-ICR MS/MS at the level of nominal mass (
i.e.
, 1 Da isolation) allowed for further estimation of the number of isomers based on unique fragmentation patterns and core fragments; the later suggested that multiple structural isomers could have very closely related CCS. These studies demonstrate the need for ultrahigh resolution TIMS mobility scan functions (
e.g.
,
R
= 200-500) in addition to tandem MS/MS isolation strategies.
In the present work, the advantages of ESI-TIMS-FT-ICR MS to address the isomeric content of dissolved organic matter are studied.