Details

Autor(en) / Beteiligte

• Zhang, Ding-kun
• Yang, Zhi-rui
• Han, Xue
• Lin, Jun-zhi
• Li, Chun-yu
• Yang, Ming
• Wang, Jia-bo
• Zhao, Yan-ling
• Xiao, Xiao-he

Titel

Microcalorimetric investigation of six alkaloids from Radix Aconite Lateralis Preparata (Fuzi) on the metabolic activity of mitochondria isolated from rat liver

Ist Teil von

• Journal of thermal analysis and calorimetry, 2017-12, Vol.130 (3), p.1707-1715

Ort / Verlag

Dordrecht: Springer Netherlands

Erscheinungsjahr

2017

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Using the TAM air isothermal microcalorimeter, the thermogenic power–time (HP–time) curves of isolated mitochondrial energy metabolism were measured, and the effects of six alkaloids from Radix Aconite Lateralis Preparata (Fuzi) including mesaconitine, aconitine, hypaconitine, benzoylmesaconine, benzoylaconine, and benzoylhypaconine on mitochondrial energy metabolism were investigated. Some quantitative information, such as k , P max , T max , Q , P av , and T lag , was obtained from the HP–time curves. The results revealed that mesaconitine, aconitine, and hypaconitine had inhibitory effect on rat-isolated mitochondrial energy metabolism, and their IC 50 value was 1.82, 2.22, and 2.48 μg mL −1 , respectively, while benzoylmesaconine, benzoylaconine, and benzoylhypaconine promoted mitochondrial energy metabolism, and their EC 50 value was 30.95, 14.36, and more than 30 μg mL −1 , respectively. Further structure–activity relationship analysis showed that the ester bond in C(8) position, hydroxyl in C(3) position, and ethyl in N atom could significantly influence the energy metabolism. These results provide scientific basis for the treatment of mitochondrial abnormalities and energy dysfunction diseases using Fuzi, and it will be of great interest to doctors and pharmacists.