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On the role of H3.3 in retroviral silencing/Elsässer et al. reply
Ist Teil von
Nature (London), 2017-08, Vol.548 (7665), p.E1
Ort / Verlag
London: Nature Publishing Group
Erscheinungsjahr
2017
Link zum Volltext
Beschreibungen/Notizen
On the basis of biochemical evidence from histone H3.3-knockout embryonic stem (ES) cells, Elsässer et al.1 reported that histone H3.3 is deposited at KAP1SETDBl-targeted ERVs by the chaperone DAXX-ATRX complex and that this deposition is required to repress ERV transcription and retrotransposition. However, our re-analysis of the published data revealed little evidence of genome-wide ERV upregulation in H3.3-knockout ES cells, and, more importantly, that the ES cells used for the analysis include polymorphic ERV insertions, which probably reflect a mixed genetic background and compromises their use for ERV expression and re-integration analysis.