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Production and characterization of surfactant-stable fungal keratinase from Gibberella intermedia CA3-1 with application potential in detergent industry
Ist Teil von
Chemical papers, 2016-11, Vol.70 (11), p.1460-1470
Ort / Verlag
Cham: Springer International Publishing
Erscheinungsjahr
2016
Link zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
Surfactant-stable keratinases with good properties are promising candidates for extensive applications in detergent industries. A novel fungal keratinase-producing strain,
Gibberella intermedia
CA3-1, is described in this study. The keratinase production medium was optimized and composed of 10 g L
−1
of wool powder, 5 g L
−1
of tryptone, 10 g L
−1
of maltodextrin and 0.5 g L
−1
of NaCl. Keratinase activity was increased up to 109 U mL
−1
from 15 U mL
−1
by culture optimization. The optimal reaction pH and temperature of the enzyme were 9.0 and 60°C, respectively. The keratinase activity could be improved by sodium dodecyl sulphate (SDS), and it remained stable in the presence of several surfactants and commercial detergents.
G. intermedia
keratinase was proved to completely remove blood stains from cotton cloth when combined with detergents. These findings indicate that this fungal keratinase is a promising catalyst for the application in detergent industry. To our knowledge, this is the first report on keratinase production by
Gibberella
genus.