Details

Autor(en) / Beteiligte

• Moreno-Contreras, Joaquín
• Espinoza, Marco A
• Sandoval-Jaime, Carlos
• Cantú-Cuevas, Marco A
• Madrid-González, Daniel A
• Barón-Olivares, Héctor
• Ortiz-Orozco, Oscar D
• Muñoz-Rangel, Asunción V
• Guzmán-Rodríguez, Cecilia
• Hernández-de la Cruz, Manuel
• Eroza-Osorio, César M
• Arias, Carlos F
• López, Susana
• Purohit, Purvi

Titel

Pooling saliva samples as an excellent option to increase the surveillance for SARS-CoV-2 when re-opening community settings

Ist Teil von

• PloS one, 2022-01, Vol.17 (1), p.e0263114-e0263114

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2022

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• In many countries a second wave of infections caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has occurred, triggering a shortage of reagents needed for diagnosis and compromising the capacity of laboratory testing. There is an urgent need to develop methods to accelerate the diagnostic procedures. Pooling samples represents a strategy to overcome the shortage of reagents, since several samples can be tested using one reaction, significantly increasing the number and speed with which tests can be carried out. We have reported the feasibility to use a direct lysis procedure of saliva as source for RNA to SARS-CoV-2 genome detection by reverse transcription quantitative-PCR (RT-qPCR). Here, we show that the direct lysis of saliva pools, of either five or ten samples, does not compromise the detection of viral RNA. In addition, it is a sensitive, fast, and inexpensive method that can be used for massive screening, especially considering the proximity of the reincorporation of activities in universities, offices, and schools.