Details

Autor(en) / Beteiligte

• Indenbaum, Victoria
• Koren, Ravit
• Katz-Likvornik, Shiri
• Yitzchaki, Mayan
• Halpern, Osnat
• Regev-Yochay, Gili
• Cohen, Carmit
• Biber, Asaf
• Feferman, Tali
• Cohen Saban, Noy
• Dhan, Roni
• Levin, Tal
• Gozlan, Yael
• Weil, Merav
• Mor, Orna
• Mandelboim, Michal
• Sofer, Danit
• Mendelson, Ella
• Lustig, Yaniv
• van Zelm, Menno C.

Titel

Testing IgG antibodies against the RBD of SARS-CoV-2 is sufficient and necessary for COVID-19 diagnosis

Ist Teil von

• PloS one, 2020-11, Vol.15 (11), p.e0241164-e0241164

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2020

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The COVID-19 pandemic and the fast global spread of the disease resulted in unprecedented decline in world trade and travel. A critical priority is, therefore, to quickly develop serological diagnostic capacity and identify individuals with past exposure to SARS-CoV-2. In this study serum samples obtained from 309 persons infected by SARS-CoV-2 and 324 of healthy, uninfected individuals as well as serum from 7 COVID-19 patients with 4-7 samples each ranging between 1-92 days post first positive PCR were tested by an "in house" ELISA which detects IgM, IgA and IgG antibodies against the receptor binding domain (RBD) of SARS-CoV-2. Sensitivity of 47%, 80% and 88% and specificity of 100%, 98% and 98% in detection of IgM, IgA and IgG antibodies, respectively, were observed. IgG antibody levels against the RBD were demonstrated to be up regulated between 1-7 days after COVID-19 detection, earlier than both IgM and IgA antibodies. Study of the antibody kinetics of seven COVID 19 patients revealed that while IgG levels are high and maintained for at least 3 months, IgM and IgA levels decline after a 35-50 days following infection. Altogether, these results highlight the usefulness of the RBD based ELISA, which is both easy and cheap to prepare, to identify COVID-19 patients even at the acute phase. Most importantly our results demonstrate that measuring IgG levels alone is both sufficient and necessary to diagnose past exposure to SARS-CoV-2.