PloS one, 2014-11, Vol.9 (11), p.e113143
2014
Details
- Autor(en) / Beteiligte
- Titel
- Efficient immuno-modulation of TH1/TH2 biomarkers in 2,4-dinitrofluorobenzene-induced atopic dermatitis: nanocarrier-mediated transcutaneous co-delivery of anti-inflammatory and antioxidant drugs
- Ist Teil von
- PloS one, 2014-11, Vol.9 (11), p.e113143
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2014
- Link zum Volltext
- Quelle
- EZB Electronic Journals Library
- Beschreibungen/Notizen
- The present study was conducted with the aim to investigate the immuno-modulatory and histological stabilization effects of nanocarrier-based transcutaneous co-delivery of hydrocortisone (HC) and hydroxytyrosol (HT). In this investigation, the clinical and pharmacological efficacies of nanoparticle (NP)-based formulation to alleviate 2,4-dinitrofluorobenzene (DNFB)-induced atopic dermatitis (AD) was explored by using an NC/Nga mouse model. Ex vivo visual examination of AD induction in experimental mice indicated remarkable control of NP-based formulations in reducing pathological severity of AD-like skin lesions. Therapeutic effectiveness of NP-based formulations was also evaluated by comparing skin thickness of AD-induced NP-treated mice (456±27 µm) with that of atopic mice (916±37 µm). Analysis of the immuno-spectrum of AD also revealed the dominance of NP-based formulations in restraining immunoglobulin-E (IgE), histamine, prostaglandin-E2 (PGE2), vascular endothelial growth factor-α (VEGF-α), and T-helper cells (TH1/TH2) producing cytokines in serum and skin biopsies of tested mice. These anti-AD data were further supported by histological findings that revealed alleviated pathological features, including collagen fiber deposition, fibroblasts infiltration, and fragmentation of elastic fibers in experimental mice. Thus, NP-mediated transcutaneous co-delivery of HC and HT can be considered as a promising therapy for managing immunological and histological spectra associated with AD.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0113143Titel-ID: cdi_plos_journals_1979937702
- Format
- –
- Schlagworte
- Administration, Cutaneous, Animals, Anti-Inflammatory Agents - administration & dosage, Antioxidants, Antioxidants - administration & dosage, Atopic dermatitis, Biology and Life Sciences, Biomarkers, Biomarkers - metabolism, Chemicals, Collagen, Cytokines, Cytokines - blood, Dermatitis, Dermatitis, Atopic - chemically induced, Dermatitis, Atopic - drug therapy, Dermatitis, Atopic - pathology, Dinitrofluorobenzene, Dinitrofluorobenzene - toxicity, Dinoprostone - analysis, Dinoprostone - blood, Disease Models, Animal, Drug Carriers - chemistry, Drug delivery systems, Drugs, Eczema, Fibers, Fibroblasts, Formulations, Helper cells, Histamine, Histamine - analysis, Histamine - blood, Hydrocortisone, Hydrocortisone - administration & dosage, Immunoglobulin E, Immunoglobulin E - analysis, Immunoglobulin E - blood, Immunology, Infiltration, Inflammation, Lesions, Lipids, Lymphocytes T, Medicine and Health Sciences, Mice, Nanoparticles, Nanoparticles - chemistry, Pathogenesis, Pharmacology, Pharmacy, Phenylethyl Alcohol - administration & dosage, Phenylethyl Alcohol - analogs & derivatives, Prostaglandin E2, Rheology, Skin, Skin - pathology, Skin diseases, Skin tests, Th1 Cells - immunology, Th1 Cells - metabolism, Th2 Cells - immunology, Th2 Cells - metabolism, Tumor necrosis factor-TNF, Vascular endothelial growth factor, Vascular Endothelial Growth Factor A - analysis, Vascular Endothelial Growth Factor A - blood