PloS one, 2017-07, Vol.12 (7), p.e0181732-e0181732
2017
Details
- Autor(en) / Beteiligte
- Titel
- Formation of singlet oxygen by decomposition of protein hydroperoxide in photosystem II
- Ist Teil von
- PloS one, 2017-07, Vol.12 (7), p.e0181732-e0181732
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2017
- Link zum Volltext
- Quelle
- EZB Electronic Journals Library
- Beschreibungen/Notizen
- Singlet oxygen (1O2) is formed by triplet-triplet energy transfer from triplet chlorophyll to O2 via Type II photosensitization reaction in photosystem II (PSII). Formation of triplet chlorophyll is associated with the change in spin state of the excited electron and recombination of triplet radical pair in the PSII antenna complex and reaction center, respectively. Here, we have provided evidence for the formation of 1O2 by decomposition of protein hydroperoxide in PSII membranes deprived of Mn4O5Ca complex. Protein hydroperoxide is formed by protein oxidation initiated by highly oxidizing chlorophyll cation radical and hydroxyl radical formed by Type I photosensitization reaction. Under highly oxidizing conditions, protein hydroperoxide is oxidized to protein peroxyl radical which either cyclizes to dioxetane or recombines with another protein peroxyl radical to tetroxide. These highly unstable intermediates decompose to triplet carbonyls which transfer energy to O2 forming 1O2. Data presented in this study show for the first time that 1O2 is formed by decomposition of protein hydroperoxide in PSII membranes deprived of Mn4O5Ca complex.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0181732Titel-ID: cdi_plos_journals_1922176420
- Format
- –
- Schlagworte
- Active oxygen, Bioenergetics, Biology and Life Sciences, Biophysics, Carbonyl compounds, Carbonyls, Chlorophyll, Chlorophyll - metabolism, Cytochrome, Decomposition, Electron spin, Electron Spin Resonance Spectroscopy - methods, Energy transfer, Energy Transfer - physiology, Engineering and Technology, Fourier transforms, Hydrogen Peroxide - metabolism, Hydroxyl radicals, Intermediates, Light, Light-Harvesting Protein Complexes - metabolism, Lipids, Membranes, Oxidation, Oxidation-Reduction, Oxygen, Oxygen - metabolism, Peroxides - metabolism, Photochemistry, Photosensitization, Photosystem, Photosystem II, Photosystem II Protein Complex - metabolism, Physical Sciences, Proteins, Recombination, Research and Analysis Methods, Singlet oxygen, Singlet Oxygen - metabolism, Spectrum analysis