PloS one, 2017-06, Vol.12 (6), p.e0179342-e0179342
2017
Details
- Autor(en) / Beteiligte
- Titel
- Focal induction of ROS-release to trigger local vascular degeneration
- Ist Teil von
- PloS one, 2017-06, Vol.12 (6), p.e0179342-e0179342
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2017
- Link zum Volltext
- Quelle
- EZB Electronic Journals Library
- Beschreibungen/Notizen
- Reactive oxygen species (ROS) play an important role in the process of cardiovascular degeneration. We evaluated the potential of a controlled, local induction of ROS-release by application of rose bengal (RB) and photo energy to induce atherosclerosis-like focal vascular degeneration in vivo. After injection of RB, rats fed with a pro-degenerative diet underwent focal irradiation of the abdominal aorta by a green laser (ROS group), while the controls received irradiation without RB. Aortic tissue was analyzed by histology and immunohistochemistry at 0, 2, 4, 8, 28 and 56 days (n = 5). The intimal surface topography was analyzed by scanning electron microscopy. In the ROS group, an initial thrombus formation had disappeared by day 8. Similarly, ROS-derived products displayed the highest concentrations at day 0. Relative matrix metalloproteinase (MMP) activity achieved a maximum after 8 days (ROS group vs. 1.60 ± 0.11 vs. 0.98 ± 0.01; p < 0.001). After 28 days, no significant differences in any aspect were found between the ROS group and the controls. However, after 56 days, the aortic tissue of ROS animals exhibited relative media-pronounced thickening (ROS vs. 2.15 ± 0.19 vs. 0.87 ± 0.10; p < 0.001) with focal calcification and reduced expression of alpha smooth muscle actin (aSMA). The ROS-releasing application of RB and photo energy allowed for the induction of vascular degeneration in a rodent model. This protocol may be used for the focal induction of vascular disease without systemic side effects and can thereby elucidate the role of ROS in the multifactorial processes of vessel degeneration and atherogenesis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0179342Titel-ID: cdi_plos_journals_1909717789
- Format
- –
- Schlagworte
- Actin, Actins - metabolism, Animals, Aorta, Aorta, Abdominal - metabolism, Aorta, Abdominal - pathology, Aorta, Abdominal - radiation effects, Apoptosis, Arteriosclerosis, Atherogenesis, Atherosclerosis, Biology and Life Sciences, Blood circulation disorders, Blood clots, Calcification, Calcification (ectopic), Calcium - blood, Caspase 3 - metabolism, Cholesterol - blood, Coronary vessels, Degeneration, Diet, Electron microscopy, Energy, Engineering and Technology, Heart surgery, Histology, Hospitals, Immunohistochemistry, Injection, Irradiation, Laboratory animals, Lasers, Male, Matrix metalloproteinase, Matrix Metalloproteinases - metabolism, Media, Medicine and Health Sciences, Metalloproteinase, Microscopy, Electron, Scanning, Muscle, Smooth - chemistry, Oxidative stress, Oxygen, Phosphates - blood, Rats, Rats, Wistar, Reactive oxygen species, Reactive Oxygen Species - chemistry, Reactive Oxygen Species - metabolism, Releasing, Research and Analysis Methods, Retinoblastoma protein, Rodents, Rose Bengal - chemistry, Scanning electron microscopy, Short term, Side effects, Smooth muscle, Thickening, Thrombosis, Topography, Triglycerides - blood, Tunica Intima - metabolism, Tunica Intima - radiation effects, Tunica Intima - ultrastructure, Vascular diseases, Vascular Diseases - blood, Vascular Diseases - metabolism