Details
- Autor(en) / Beteiligte
- Titel
- Silencing of the hTERT gene by shRNA inhibits colon cancer SW480 cell growth in vitro and in vivo
- Ist Teil von
- PloS one, 2014-09, Vol.9 (9), p.e107019
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2014
- Link zum Volltext
- Quelle
- Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
- Beschreibungen/Notizen
- Human telomerase reverse transcriptase (hTERT) is the key enzyme responsible for synthesizing and maintaining the telomeres on the ends of chromosomes, and it is essential for cell proliferation. This has made hTERT a focus of oncology research and an attractive target for anticancer drug development. In this study, we designed a small interfering RNA (siRNA) targeting the catalytic subunit of hTERT and tested its effects on the growth of telomerase-positive human colon carcinoma SW480 cells in vitro, as well as on the tumorigenicity of these cells in nude mice. Transient and stable transfection of hTERT siRNA into colon cancer SW480 cells suppressed hTERT expression, reduced telomerase activity and inhibited cell growth and proliferation. Knocking down hTERT expression in SW480 tumors xenografted into nude mice significantly slowed tumor growth and promoted tumor cell apoptosis. Our results suggest that hTERT is involved in carcinogenesis of human colon carcinoma, and they highlight the therapeutic potential of a hTERT knock-down approach.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0107019Titel-ID: cdi_plos_journals_1561282708
- Format
- –
- Schlagworte
- Animals, Apoptosis, Apoptosis - genetics, Biology and Life Sciences, Cancer, Cancer therapies, Carcinogenesis, Carcinogens, Carcinoma - enzymology, Carcinoma - genetics, Carcinoma - pathology, Carcinoma - therapy, Catalysis, Cell growth, Cell Line, Tumor, Cell Proliferation, Chromosomes, Colon, Colon cancer, Colonic Neoplasms - enzymology, Colonic Neoplasms - genetics, Colonic Neoplasms - pathology, Colonic Neoplasms - therapy, Colorectal cancer, Drug development, Endoscopy, Enzymes, Gene Expression Regulation, Neoplastic, Gene therapy, Genetic Therapy - methods, Hospitals, Humans, Laboratory animals, Medicine and Health Sciences, Mice, Mice, Nude, Neoplasm Transplantation, Research and Analysis Methods, Ribonucleic acid, RNA, RNA, Small Interfering - genetics, RNA, Small Interfering - metabolism, RNA-directed DNA polymerase, siRNA, Telomerase, Telomerase - antagonists & inhibitors, Telomerase - genetics, Telomerase - metabolism, Telomerase reverse transcriptase, Telomere Homeostasis, Telomeres, Transfection, Tumorigenicity, Tumors, Xenografts