PloS one, 2013-07, Vol.8 (7), p.e66391-e66391
2013
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- Autor(en) / Beteiligte
- Titel
- Carvedilol decrease IL-1β and TNF-α, inhibits MMP-2, MMP-9, COX-2, and RANKL expression, and up-regulates OPG in a rat model of periodontitis
- Ist Teil von
- PloS one, 2013-07, Vol.8 (7), p.e66391-e66391
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Periodontal diseases are initiated primarily by Gram-negative, tooth-associated microbial biofilms that elicit a host response that causes osseous and soft tissue destruction. Carvedilol is a β-blocker used as a multifunctional neurohormonal antagonist that has been shown to act not only as an anti-oxidant but also as an anti-inflammatory drug. This study evaluated whether Carvedilol exerted a protective role against ligature-induced periodontitis in a rat model and defined how Carvedilol affected metalloproteinases and RANKL/RANK/OPG expression in the context of bone remodeling. Rats were randomly divided into 5 groups (n = 10/group): (1) non-ligated (NL), (2) ligature-only (LO), and (3) ligature plus Carvedilol (1, 5 or 10 mg/kg daily for 10 days). Periodontal tissue was analyzed for histopathlogy and using immunohistochemical analysis characterized the expression profiles of MMP-2, MMP-9, COX-2, and RANKL/RANK/OPG and determined the presence of IL-1β, IL-10 and TNF-α, myeloperoxidase (MPO), malonaldehyde (MDA) and, glutathione (GSH). MPO activity in the group with periodontal disease was significantly increased compared to the control group (p<0.05). Rats treated with 10 mg/kg Carvedilol presented with significantly reduced MPO and MDA concentrations (p<0.05) in addition to presenting with reduced levels of the pro-inflammatory cytokines IL-1 β and TNF-α (p<0.05). IL-10 levels in Carvedilol-treated rats remained unaltered. Immunohistochemical analysis demonstrated reduced expression of MMP-2, MMP-9, RANK, RANKL, COX-2, and OPG in rats treated with 10 mg/kg Carvedilol. This study demonstrated that Carvedilol affected bone formation/destruction and anti-inflammatory activity in a rat model of periodontitis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0066391Titel-ID: cdi_plos_journals_1382396502
- Format
- –
- Schlagworte
- Animals, Anti-inflammatory agents, Antioxidants, Atherosclerosis, Biofilms, Biology, Bone growth, Bone remodeling, Bone remodelling, Carbazoles - administration & dosage, Carbazoles - pharmacology, Cyclooxygenase 2 - metabolism, Cyclooxygenase-2, Cytokines, Dental research, Destruction, Disease control, Disease Models, Animal, Enzymes, Gelatinase A, Gelatinase B, Glutathione, Gum disease, Heart failure, Inflammation, Interleukin 1, Interleukin 10, Interleukin-1beta - metabolism, Male, Malondialdehyde, Matrix Metalloproteinase 2 - metabolism, Matrix Metalloproteinase 9 - metabolism, Medicine, Microorganisms, Microscopy, Morphology, Neutrophils, Osteogenesis, Osteoprotegerin, Osteoprotegerin - metabolism, Oxidative Stress, Periodontal disease, Periodontal diseases, Periodontitis, Periodontitis - genetics, Periodontitis - metabolism, Periodontitis - pathology, Peroxidase, Propanolamines - administration & dosage, Propanolamines - pharmacology, Public health, RANK Ligand - metabolism, Rats, Rodents, Teeth, TRANCE protein, Tumor Necrosis Factor-alpha - metabolism, Tumor necrosis factor-TNF, Tumor necrosis factor-α