PloS one, 2012-11, Vol.7 (11), p.e48582
2012
Details
- Autor(en) / Beteiligte
- Titel
- Suprabasin is hypomethylated and associated with metastasis in salivary adenoid cystic carcinoma
- Ist Teil von
- PloS one, 2012-11, Vol.7 (11), p.e48582
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2012
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Salivary gland adenoid cystic carcinoma (ACC) is a rare cancer, accounting for only 1% of all head and neck malignancies. ACC is well known for perineural invasion and distant metastasis, but its underlying molecular mechanisms of carcinogenesis are still unclear. Here, we show that a novel oncogenic candidate, suprabasin (SBSN), plays important roles in maintaining the anchorage-independent and anchorage-dependent cell proliferation in ACC by using SBSN shRNA stably transfected ACC cell line clones. SBSN is also important in maintaining the invasive/metastatic capability in ACC by Matrigel invasion assay. More interestingly, SBSN transcription is significantly upregulated by DNA demethylation induced by 5-aza-2'-deoxycytidine plus trichostatin A treatment and the DNA methylation levels of the SBSN CpG island located in the second intron were validated to be significantly hypomethylated in primary ACC samples versus normal salivary gland tissues. Taken together, these results support SBSN as novel oncogene candidate in ACC, and the methylation changes could be a promising biomarker for ACC.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0048582Titel-ID: cdi_plos_journals_1326737951
- Format
- –
- Schlagworte
- Adenoid, Adolescent, Adult, Aged, Aged, 80 and over, Antigens, Differentiation - genetics, Antigens, Differentiation - metabolism, Bioindicators, Biology, Biomarkers, Cancer, Cancer metastasis, Carcinogenesis, Carcinogens, Carcinoma, Carcinoma, Adenoid Cystic - genetics, Carcinoma, Adenoid Cystic - pathology, Cell Adhesion, Cell Proliferation, CpG islands, CpG Islands - genetics, Cyclin-dependent kinases, Demethylation, Deoxyribonucleic acid, Development and progression, DNA, DNA methylation, DNA Methylation - genetics, Epigenetics, Female, Gene expression, Gene Expression Regulation, Neoplastic, Genetic transcription, Head, Humans, Invasiveness, Kinases, Male, Medicine, Metastases, Metastasis, Methylation, Middle Aged, Molecular modelling, Neck, Neoplasm Invasiveness, Neoplasm Metastasis, Neoplasm Proteins - genetics, Neoplasm Proteins - metabolism, Otolaryngology, Salivary gland, Salivary Gland Neoplasms - genetics, Salivary Gland Neoplasms - pathology, Salivary Glands - metabolism, Salivary Glands - pathology, Stem cells, Surgery, Tissues, Transcription, Transcription (Genetics), Trichostatin A, Up-Regulation - genetics, Young Adult