PloS one, 2012-02, Vol.7 (2), p.e29552-e29552
2012
Details
- Autor(en) / Beteiligte
- Titel
- Immunization of mice with recombinant protein CobB or AsnC confers protection against Brucella abortus infection
- Ist Teil von
- PloS one, 2012-02, Vol.7 (2), p.e29552-e29552
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2012
- Link zum Volltext
- Quelle
- EZB Electronic Journals Library
- Beschreibungen/Notizen
- Due to drawbacks of live attenuated vaccines, much more attention has been focused on screening of Brucella protective antigens as subunit vaccine candidates. Brucella is a facultative intracellular bacterium and cell mediated immunity plays essential roles for protection against Brucella infection. Identification of Brucella antigens that present T-cell epitopes to the host could enable development of such vaccines. In this study, 45 proven or putative pathogenesis-associated factors of Brucella were selected according to currently available data. After expressed and purified, 35 proteins were qualified for analysis of their abilities to stimulate T-cell responses in vitro. Then, an in vitro gamma interferon (IFN-γ) assay was used to identify potential T-cell antigens from B. abortus. In total, 7 individual proteins that stimulated strong IFN-γ responses in splenocytes from mice immunized with B. abortus live vaccine S19 were identified. The protective efficiencies of these 7 recombinant proteins were further evaluated. Mice given BAB1_1316 (CobB) or BAB1_1688 (AsnC) plus adjuvant could provide protection against virulent B. abortus infection, similarly with the known protective antigen Cu-Zn SOD and the license vaccine S19. In addition, CobB and AsnC could induce strong antibodies responses in BALB/c mice. Altogether, the present study showed that CobB or AsnC protein could be useful antigen candidates for the development of subunit vaccines against brucellosis with adequate immunogenicity and protection efficacy.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0029552Titel-ID: cdi_plos_journals_1323855120
- Format
- –
- Schlagworte
- Amino acids, Analysis, Animals, Antibodies, Antigenic determinants, Antigens, Antigens, Bacterial - immunology, AsnC protein, Biological response modifiers, Biology, Brucella abortus, Brucella abortus - metabolism, Brucella Vaccine - immunology, Brucellosis, Brucellosis - immunology, Brucellosis - prevention & control, Disease control, Disease prevention, Education, Enzymes, Epitopes, Escherichia coli Proteins - metabolism, Female, Health aspects, Identification, Immune System, Immunity, Immunization, Immunogenicity, Infection, Infections, Infectious diseases, Interferon, Interferon-gamma - metabolism, Laboratories, Lymphocytes B, Lymphocytes T, Medical research, Medicine, Medicine, Experimental, Metabolism, Mice, Mice, Inbred BALB C, Mycobacterium avium, Mycobacterium tuberculosis, Pathogenesis, Protective antigen, Proteins, Proteomics, Public health, Recombinant proteins, Recombinant Proteins - metabolism, Science, Sirtuins - metabolism, Spleen - cytology, Splenocytes, Superoxide Dismutase - metabolism, T cells, T-Lymphocytes - metabolism, T-Lymphocytes - microbiology, Trans-Activators - metabolism, Tuberculosis, Vaccines, Veterinary colleges, Veterinary medicine, Veterinary Science, Virulence (Microbiology), Yersinia pestis, Zinc, Zoonoses, γ-Interferon