PloS one, 2011-04, Vol.6 (4), p.e19087-e19087
2011
Details
- Autor(en) / Beteiligte
- Titel
- A phagocytic route for uptake of double-stranded RNA in RNAi
- Ist Teil von
- PloS one, 2011-04, Vol.6 (4), p.e19087-e19087
- Ort / Verlag
- United States: Public Library of Science
- Erscheinungsjahr
- 2011
- Link zum Volltext
- Quelle
- EZB Free E-Journals
- Beschreibungen/Notizen
- RNA interference (RNAi) has a range of physiological functions including as a defence mechanism against viruses. To protect uninfected cells in a multicellular organism, not only a cell-autonomous RNAi response is required but also a systemic one. However, the route of RNA spread in systemic RNAi remains unclear. Here we show that phagocytosis can be a route for double-stranded RNA uptake. Double-stranded RNA expressed in Escherichia coli induces robust RNAi in Drosophila S2 cells, with effectiveness comparable to that of naked dsRNA. We could separate this phagocytic uptake route from that for RNAi induced by naked dsRNA. Therefore, phagocytic uptake of dsRNA offers a potential route for systemic spread of RNAi.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1932-6203eISSN: 1932-6203DOI: 10.1371/journal.pone.0019087Titel-ID: cdi_plos_journals_1297624966
- Format
- –
- Schlagworte
- Animals, Bacteria, Base Sequence, Biology, Carrier Proteins - metabolism, Cell Line, Clathrin - chemistry, Cloning, Cytochalasin D - pharmacology, Double-stranded RNA, Drosophila, Drosophila melanogaster, Drosophila Proteins - metabolism, E coli, Endocytosis, Escherichia coli, Escherichia coli - genetics, Gene expression, Genes, Insects, Kinases, Laboratories, Molecular Sequence Data, Nucleic Acid Synthesis Inhibitors - pharmacology, Phagocytes, Phagocytosis, Physiological aspects, Proteins, Ribonuclease III - metabolism, Ribonucleic acid, RNA, RNA Interference, RNA polymerase, RNA, Double-Stranded - genetics, RNA-mediated interference, Rodents, Viruses