Details

Autor(en) / Beteiligte

• Yamada, Shinya
• Hatta, Masato
• Staker, Bart L
• Watanabe, Shinji
• Imai, Masaki
• Shinya, Kyoko
• Sakai-Tagawa, Yuko
• Ito, Mutsumi
• Ozawa, Makoto
• Watanabe, Tokiko
• Sakabe, Saori
• Li, Chengjun
• Kim, Jin Hyun
• Myler, Peter J
• Phan, Isabelle
• Raymond, Amy
• Smith, Eric
• Stacy, Robin
• Nidom, Chairul A
• Lank, Simon M
• Wiseman, Roger W
• Bimber, Benjamin N
• O'Connor, David H
• Neumann, Gabriele
• Stewart, Lance J
• Kawaoka, Yoshihiro
• Perez, Daniel R.

Titel

Biological and structural characterization of a host-adapting amino acid in influenza virus

Ist Teil von

• PLoS pathogens, 2010-08, Vol.6 (8), p.e1001034-e1001034

Ort / Verlag

United States: Public Library of Science

Erscheinungsjahr

2010

Link zum Volltext

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• Two amino acids (lysine at position 627 or asparagine at position 701) in the polymerase subunit PB2 protein are considered critical for the adaptation of avian influenza A viruses to mammals. However, the recently emerged pandemic H1N1 viruses lack these amino acids. Here, we report that a basic amino acid at position 591 of PB2 can compensate for the lack of lysine at position 627 and confers efficient viral replication to pandemic H1N1 viruses in mammals. Moreover, a basic amino acid at position 591 of PB2 substantially increased the lethality of an avian H5N1 virus in mice. We also present the X-ray crystallographic structure of the C-terminus of a pandemic H1N1 virus PB2 protein. Arginine at position 591 fills the cleft found in H5N1 PB2 proteins in this area, resulting in differences in surface shape and charge for H1N1 PB2 proteins. These differences may affect the protein's interaction with viral and/or cellular factors, and hence its ability to support virus replication in mammals.