Details

Autor(en) / Beteiligte

• Lu, Wen-Quan
• Qiu, Yan
• Li, Tie-Jun
• Tao, Xia
• Sun, Lian-Na
• Chen, Wan-Sheng

Titel

Timosaponin B-II inhibits pro-inflammatory cytokine induction by lipopolysaccharide in BV2 cells

Ist Teil von

• Archives of Pharmacal Research, 2009, 32(9), , pp.1301-1308

Ort / Verlag

Heidelberg: Pharmaceutical Society of Korea

Erscheinungsjahr

2009

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• It was reported that the total polysaccharides extracts from Anemarrhenae asphodeloides Bge (Liliaceae, rhizome) could inhibit inflammatory responses in various models. In the present study, the effects of Timosaponin B-II, a purified extract from A. asphodeloidesb , on the expressions of IL-1β, TNF-α and IL-6, the activity of NF-κB and the activation of signal pathway related to NF-κB were explored in vitro . Timosaponin B-II significantly attenuated increase of these cytokines on both mRNA and protein levels from LPS-stimulated BV2 cells in a dose-dependent manner. The reporter gene assay also showed that the activation of NF-κB induced by LPS was inhibited by pre-treatment with Timosaponin B-II. Moreover, western blot results showed that the activation of p38, JNK and P65 had been decreased. These results suggest that both NF-κB signal pathway and MAPK pathway were involved in the inhibitory effects of Timosaponin B-II on the expression of pro-inflammatory cytokines.