Details

Autor(en) / Beteiligte

• Tang, S.‐H.
• Wang, H.‐Y.
• Sun, H.
• An, N.
• Xiao, L.
• Sun, Q.
• Zhao, D.‐B.

Titel

Propofol inhibits carbachol‐induced chloride secretion by directly targeting the basolateral K+ channel in rat ileum epithelium

Ist Teil von

• Neurogastroenterology and motility, 2017-02, Vol.29 (2), p.n/a

Ort / Verlag

England: Wiley Subscription Services, Inc

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• Background Propofol is a widely used intravenous general anesthetic. Acetylcholine (ACh) is critical in controlling epithelial ion transport. This study was to investigate the effects of propofol on ACh‐evoked secretion in rat ileum epithelium. Methods The Ussing chamber technique was used to investigate the effects of propofol on carbachol (CCh)‐evoked short‐circuit currents (Isc). Key Results Propofol (10−2–10−6 mol/L) attenuated CCh‐evoked Isc of rat ileum mucosa in a dose‐dependent manner. The inhibitory effect of propofol was only evident after application to the serosal side. Pretreatment with tetrodotoxin (TTX, 0.3 μmol/L, n=5) had no effect on propofol‐induced inhibitory effect, whereas serosal application of K+ channel inhibitor, glibenclamide, but not, an ATP‐sensitive K+ channel inhibitor, largely reduced the inhibitory effect of propofol. In addition, pretreatment with either hexamethonium bromide (HB, nicotinic nACh receptor antagonist) or Cl− channel blockers niflumic acid and cystic fibrosis transmembrane conductance regulator (inh)‐172 did not produce any effect on the propofol‐induced inhibitory effect. Conclusions & Inferences Propofol inhibits CCh‐induced intestinal secretion by directly targeting basolateral K+ channels. The aim of this study was to investigate the effects of propofol on carbachol (CCh)‐evoked short‐circuit currents (Isc). The results show that propofol inhibits CCh‐induced intestinal secretion by directly targeting basolateral K+ channels.