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Biodiesel is an alternative fuel that is included in renewable energy. Various problems of the source of raw materials for making biodiesel create an opportunity to find alternative sources of raw materials from plant oils, one of which is papaya seeds. Extraction of papaya seeds into oil is carried out by socletation and distillation methods. GCMS analysis showed that papaya seed oil contained oleic acid. Biodiesel transesterification has been carried out using CaO catalyst from the Strombus canarium shells. The reaction was carried out at the papaya seed oil to methanol molar ratio of 1: 6, presence catalyst 5% wt relative to oil and temperature reaction of 65 °C for 2 h. GC/MS analysis result showed that the content of methyl esters is 50.35% with the highest abundance is 10-Octadecenoic acid and the smallest abundance is 9-Octadecenoic acid.