Diagnostic microbiology and infectious disease, 2015-08, Vol.82 (4), p.289-291
2015
Details
- Autor(en) / Beteiligte
- Titel
- Molecular diagnosis of toxoplasmosis: value of the buffy coat for the detection of circulating Toxoplasma gondii
- Ist Teil von
- Diagnostic microbiology and infectious disease, 2015-08, Vol.82 (4), p.289-291
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2015
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Abstract Early detection of Toxoplasma tachyzoites circulating in blood using PCR is recommended for immunosuppressed patients at high risk for disseminated toxoplasmosis. Using a toxoplasmosis mouse model, we show that the sensitivity of detection is higher using buffy coat isolated from a large blood volume than using whole blood for this molecular monitoring.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0732-8893eISSN: 1879-0070DOI: 10.1016/j.diagmicrobio.2015.04.004Titel-ID: cdi_hal_primary_oai_HAL_hal_01262441v1
- Format
- –
- Schlagworte
- Animals, Blood Buffy Coat - parasitology, Buffy coat, Diagnosis, Disease Models, Animal, Female, Humans, Infectious Disease, Internal Medicine, Life Sciences, Mice, Molecular Diagnostic Techniques - methods, Parasitology - methods, PCR, Polymerase Chain Reaction - methods, Santé publique et épidémiologie, Sensitivity and Specificity, Toxoplasma - genetics, Toxoplasma - isolation & purification, Toxoplasma gondii, Toxoplasmosis, Toxoplasmosis - diagnosis, Toxoplasmosis - parasitology, Whole blood