Details

Autor(en) / Beteiligte

• Fukuyama, Yoshiko
• Tokuhara, Daisuke
• Sekine, Shinichi
• Aso, Kazuyoshi
• Kataoka, Kosuke
• Davydova, Julia
• Yamamoto, Masato
• Gilbert, Rebekah S
• Tokuhara, Yuka
• Fujihashi, Keiko
• Kunisawa, Jun
• Yuki, Yoshikazu
• Kiyono, Hiroshi
• McGhee, Jerry R
• Fujihashi, Kohtaro

Titel

Potential Roles of CCR5.sup.+ CCR6.sup.+ Dendritic Cells Induced by Nasal Ovalbumin plus Flt3 Ligand Expressing Adenovirus for Mucosal IgA Responses

Ist Teil von

• PloS one, 2013-04, Vol.8 (4), p.e60453

Ort / Verlag

Public Library of Science

Erscheinungsjahr

2013

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• We assessed the role of CCR5.sup.+ /CCR6.sup.+ /CD11b.sup.+ /CD11c.sup.+ dendritic cells (DCs) for induction of ovalbumin (OVA)-specific antibody (Ab) responses following mucosal immunization. Mice given nasal OVA plus an adenovirus expressing Flt3 ligand (Ad-FL) showed early expansion of CCR5.sup.+ /CCR6.sup.+ /CD11b.sup.+ /CD11c.sup.+ DCs in nasopharyngeal-associated lymphoid tissue (NALT) and cervical lymph nodes (CLNs). Subsequently, this DC subset became resident in submandibular glands (SMGs) and nasal passages (NPs) in response to high levels of CCR-ligands produced in these tissues. CD11b.sup.+ /CD11c.sup.+ DCs were markedly decreased in both CCR5.sup.-/- and CCR6.sup.-/- mice. Chimera mice reconstituted with bone marrow cells from CD11c-diphtheria toxin receptor (CD11c-DTR) and CCR5.sup.-/- or CD11c-DTR and CCR6.sup.-/- mice given nasal OVA plus Ad-FL had elevated plasma IgG, but reduced IgA as well as low anti-OVA secretory IgA (SIgA )Ab responses in saliva and nasal washes. These results suggest that CCR5.sup.+ CCR6.sup.+ DCs play an important role in the induction of Ag-specific SIgA Ab responses.