Details

Autor(en) / Beteiligte

• Yang, Yongping
• Shi, Wei
• Abiona, Olubukola M
• Nazzari, Alexandra
• Olia, Adam S
• Ou, Li
• Phung, Emily
• Stephens, Tyler
• Tsybovsky, Yaroslav
• Verardi, Raffaello
• Wang, Shuishu
• Werner, Anne
• Yap, Christina
• Ambrozak, David
• Bylund, Tatsiana
• Liu, Tracy
• Nguyen, Richard
• Wang, Lingshu
• Zhang, Baoshan
• Zhou, Tongqing
• Chuang, Gwo-Yu
• Graham, Barney S
• Mascola, John R
• Corbett, Kizzmekia S
• Kwong, Peter D

Titel

Newcastle Disease Virus-Like Particles Displaying Prefusion-Stabilized SARS-CoV-2 Spikes Elicit Potent Neutralizing Responses

Ist Teil von

• Vaccines (Basel), 2021-01, Vol.9 (2), p.73

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Electronic Journals Library - Freely accessible e-journals

Beschreibungen/Notizen

• The COVID-19 pandemic highlights an urgent need for vaccines that confer protection from SARS-CoV-2 infection. One approach to an effective COVID-19 vaccine may be through the display of SARS-CoV-2 spikes on the surface of virus-like particles, in a manner structurally mimicking spikes on a native virus. Here we report the development of Newcastle disease virus-like particles (NDVLPs) displaying the prefusion-stabilized SARS-CoV-2 spike ectodomain (S2P). Immunoassays with SARS-CoV-2-neutralizing antibodies revealed the antigenicity of S2P-NDVLP to be generally similar to that of soluble S2P, and negative-stain electron microscopy showed S2P on the NDVLP surface to be displayed with a morphology corresponding to its prefusion conformation. Mice immunized with S2P-NDVLP showed substantial neutralization titers (geometric mean ID = 386) two weeks after prime immunization, significantly higher than those elicited by a molar equivalent amount of soluble S2P (geometric mean ID = 17). Neutralizing titers at Week 5, two weeks after a boost immunization with S2P-NDVLP doses ranging from 2.0 to 250 μg, extended from 2125 to 4552, and these generally showed a higher ratio of neutralization versus ELISA than observed with soluble S2P. Overall, S2P-NDVLP appears to be a promising COVID-19 vaccine candidate capable of eliciting substantial neutralizing activity.