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In order to determine the more accurate fertile potential of sperm, it seems
that the conventional parameters of boar semen quality (the ejaculate volume,
sperm concentra?tion, progressive motility, percentage of live sperm and of
those with intact acrosomal mor?phology) are insufficient. Since recently,
there have been numerous studies proving that protein concentration in sperm
plasma has high positive correlation with boar fertile po?tential. The
research objective was to determine the effect of boars as well as the season
on the variation of protein content in the sperm plasma. For the research
there were used spermal fractions of 2 boars with high (V-boar) and 2 boars
with low (N-boar) protein con?tent in spermal plasma. The ejaculates of boars
were taken once a week, for a month, during one year (4 ? 12 = 48 ejaculates
per boar). For protein analysis in the spermal plas?ma, the samples were
prepared by centrifugation. The ejaculate volume, protein concen?tration and
progressive motility varied considerably (p < 0.05 or p < 0.01) among the
boars as well as in one and the same boar. The variations of the same
parameters were deter?mined also during both warm and cold season. On the
other hand, protein concentration was rather constant, and in V-boars (ranged
from 4 to 4.5%) while in N-boars (ranged from 2.3 to 2.6%). The season did
not significantly affect (p > 0.01) protein content in sperm plasma (V-boars:
4 to 4.5% in warm and cold season; N-boars: 2.3 do 2.6% in warm and 2.3 to
2.5% in cold season). The obtained results showed that measurement of protein
con?tent in boar sperm plasma could be a useful method for their ranking,
based on fertile po?tential of fresh semen.
nema