Nature communications, 2022-01, Vol.13 (1), p.131-131, Article 131
2022
Details
- Autor(en) / Beteiligte
- Titel
- Dynamic transcriptome and chromatin architecture in granulosa cells during chicken folliculogenesis
- Ist Teil von
- Nature communications, 2022-01, Vol.13 (1), p.131-131, Article 131
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2022
- Link zum Volltext
- Quelle
- Alma/SFX Local Collection
- Beschreibungen/Notizen
- Folliculogenesis is a complex biological process involving a central oocyte and its surrounding somatic cells. Three-dimensional chromatin architecture is an important transcription regulator; however, little is known about its dynamics and role in transcriptional regulation of granulosa cells during chicken folliculogenesis. We investigate the transcriptomic dynamics of chicken granulosa cells over ten follicular stages and assess the chromatin architecture dynamics and how it influences gene expression in granulosa cells at three key stages: the prehierarchical small white follicles, the first largest preovulatory follicles, and the postovulatory follicles. Our results demonstrate the consistency between the global reprogramming of chromatin architecture and the transcriptomic divergence during folliculogenesis, providing ample evidence for compartmentalization rearrangement, variable organization of topologically associating domains, and rewiring of the long-range interaction between promoter and enhancers. These results provide key insights into avian reproductive biology and provide a foundational dataset for the future in-depth functional characterization of granulosa cells.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2041-1723eISSN: 2041-1723DOI: 10.1038/s41467-021-27800-9Titel-ID: cdi_doaj_primary_oai_doaj_org_article_f0085c053dad475da55c2a5befc680ac
- Format
- –
- Schlagworte
- Animals, Avian Proteins - classification, Avian Proteins - genetics, Avian Proteins - metabolism, Biological activity, Chickens, Chickens - genetics, Chickens - growth & development, Chickens - metabolism, Chromatin, Chromatin - chemistry, Chromatin - ultrastructure, Enhancer Elements, Genetic, Enhancers, Female, Follicles, Folliculogenesis, Gametocytes, Gene expression, Gene Expression Profiling, Gene Expression Regulation, Developmental, Gene Ontology, Gene regulation, Granulosa cells, Granulosa Cells - cytology, Granulosa Cells - metabolism, Molecular Sequence Annotation, Oocytes - cytology, Oocytes - metabolism, Oogenesis - genetics, Poultry, Promoter Regions, Genetic, Somatic cells, Transcription, Transcriptome, Transcriptomes, Transcriptomics