Details

Autor(en) / Beteiligte

• Lima, Maria Laura de Souza
• Medeiros, Caroline Addison Carvalho Xavier de
• Guerra, Gerlane Coelho Bernardo
• Santos, Robson
• Bader, Michael
• Pirih, Flavia Q
• Araújo Júnior, Raimundo Fernandes de
• Chan, Alan B
• Cruz, Luis J
• Brito, Gerly Anne de Castro
• Leitão, Renata Ferreira de Carvalho
• Silveira, Ericka Janine Dantas da
• Garcia, Vinicius Barreto
• Martins, Agnes Andrade
• Araújo, Aurigena Antunes de

Titel

AT1 and AT2 Receptor Knockout Changed Osteonectin and Bone Density in Mice in Periodontal Inflammation Experimental Model

Ist Teil von

• International journal of molecular sciences, 2021-05, Vol.22 (10), p.5217

Ort / Verlag

Switzerland: MDPI AG

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• The aim of this study was to evaluate the role of AT1 and AT2 receptors in a periodontal inflammation experimental model. Periodontal inflammation was induced by LPS/ . Maxillae, femur, and vertebra were scanned using Micro-CT. Maxillae were analyzed histopathologically, immunohistochemically, and by RT-PCR. The vertebra showed decreased BMD in AT1 H compared with WT H ( < 0.05). The femur showed increased Tb.Sp for AT1 H and AT2 H, < 0.01 and < 0.05, respectively. The Tb.N was decreased in the vertebra (WT H-AT1 H: < 0.05; WT H-AT2 H: < 0.05) and in the femur (WT H-AT1 H: < 0.01; WT H-AT2 H: < 0.05). AT1 PD increased linear bone loss ( < 0.05) and decreased osteoblast cells ( < 0.05). RANKL immunostaining was intense for AT1 PD and WT PD ( < 0.001). OPG was intense in the WT H, WT PD, and AT2 PD when compared to AT1 PD ( < 0.001). AT1 PD showed weak immunostaining for osteocalcin compared with WT H, WT PD, and AT2 PD ( < 0.001). AT1 H showed significantly stronger immunostaining for osteonectin in fibroblasts compared to AT2 H ( < 0.01). AT1 receptor knockout changed bone density, the quality and number of bone trabeculae, decreased the number of osteoblast cells, and increased osteonectin in fibroblasts.